Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness
| Autor: | Wayne F. Patton, Brian Agnew, Satyabrata Nandi, Lakshmanswamy Rajkumar, Raphael C. Guzman, Yue Ge |
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| Rok vydání: | 2004 |
| Předmět: |
Proteomics
Glycosylation Breast Neoplasms Biology Biochemistry chemistry.chemical_compound Breast cancer medicine Animals Electrophoresis Gel Two-Dimensional Phosphorylation Molecular Biology chemistry.chemical_classification Gel electrophoresis Proteins Phosphoproteins medicine.disease Fluorescence Molecular biology Rats Disease Models Animal Electrophoresis chemistry Female Glycoprotein |
| Zdroj: | PROTEOMICS. 4:3464-3467 |
| ISSN: | 1615-9861 1615-9853 |
| DOI: | 10.1002/pmic.200400957 |
| Popis: | The Multiplexed Proteomics (MP) technology is a new approach that permits quantitative, multicolor fluorescence detection of proteins in one-dimensional or two-dimensional gels. This methodology allows for multiplexed identification and differential analysis of phosphoproteins, glycoproteins, and total proteins within a single gel electrophoresis experiment. Here the MP system was applied to the differential proteomic analysis of pregnancy-induced refractoriness to breast cancer using a rat model system. Differential analyses identified multiple proteins with altered phosphorylation, glycosylation, or protein expression patterns. |
| Databáze: | OpenAIRE |
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