Salting out assisted liquid-liquid extraction for liquid chromatography tandem-mass spectrometry determination of amphetamine-like stimulants in meconium
| Autor: | Radomír Čabala, David Sýkora, Tomáš Hložek, Alžběta Nemeškalová, Martin Kuchař, Miroslava Bursová |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Meconium
Substance-Related Disorders Methylone Clinical Biochemistry Liquid-Liquid Extraction Pharmaceutical Science Methylenedioxypyrovalerone 01 natural sciences Analytical Chemistry chemistry.chemical_compound Mephedrone Liquid chromatography–mass spectrometry Limit of Detection Pregnancy Tandem Mass Spectrometry Drug Discovery Ammonium Compounds medicine Humans Butylone Spectroscopy Chromatography High Pressure Liquid Chromatography 010405 organic chemistry 010401 analytical chemistry Amphetamines Infant Newborn Flephedrone 0104 chemical sciences Pregnancy Complications Substance Abuse Detection chemistry Naphyrone Feasibility Studies Central Nervous System Stimulants Female medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 172 |
| ISSN: | 1873-264X |
| Popis: | In the last decade there has been a dramatic increase in the availability and abuse of synthetic cathinones – new amphetamine-like stimulants. Even though their abuse during pregnancy could have serious adverse effects on the fetus, cathinones are not readily included in neonatal toxicological screenings. Meconium (first neonatal stool) is the specimen of choice to reveal long term drug exposure, however as it is a highly complex matrix, the sample preparation is a critical step before the instrumental analysis. The aim of this work was to develop a suitable meconium sample extraction technique using the advantages of salting-out assisted liquid-liquid extraction (SALLE) and using only MS-friendly organic ammonium salts. We further developed and validated liquid chromatography tandem-mass spectrometry method for the determination of ‘traditional’ stimulants (methamphetamine, amphetamine, MDMA) and cathinones (mephedrone, methylenedioxypyrovalerone (MDPV), α-pyrrolidinopentiophenone (α-PVP), methylone, butylone, flephedrone, and naphyrone). Matrix-matched calibration was prepared in the concentration range 10–2000 ng/g. The limits of quantification were determined as 10 ng/g, recoveries ranged from 48.2% to 94.3% and the matrix effect was between 60.2% and 101.4%. Accuracy (86.1–114.5%) and precision (4.9–14.9%) were determined and all validation criteria were met for all analytes except for naphyrone. Finally, our analytical method was tested on a set of real meconium samples, which were found positive for amphetamine, methamphetamine and methylone, thus demonstrating the validity of the method. |
| Databáze: | OpenAIRE |
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