Establishment of a ciliated epithelial cell line from human Fallopian tube
| Autor: | Shigehiko Mizutani, Shigeru Toda, Fumitaka Kikkawa, Hisao Ando, Tetsuo Masahashi, Miya Kobayashi |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
animal structures Liver cytology Cytological Techniques Biology Embryonic and Fetal Development Internal medicine medicine Humans Cilia Blastocyst Interleukin 8 Fallopian Tubes Cell Line Transformed Rehabilitation Obstetrics and Gynecology Epithelial Cells Embryo Mammalian Molecular biology Coculture Techniques Epithelium medicine.anatomical_structure Endocrinology Liver Reproductive Medicine Cell culture embryonic structures Cytokines Oviduct Female Leukemia inhibitory factor Fallopian tube |
| Zdroj: | Human Reproduction. 15:1597-1603 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme. All of the 133 embryos had >/=10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10-30% fragments reached the blastocyst stage by co-culture. In contrast, blastocyst formation was observed in |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|