DNA methylation profiles of immune response-related genes in apical periodontitis
| Autor: | Ariadne Letra, K. Maheshwari, Talita Tartari, L. C. Souza, C. Wichnieski, Gustavo Pompermaier Garlet, Everdan Carneiro, F. Nieves, Renato Menezes Silva |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Adolescent Periodontal Ligament Fas-Associated Death Domain Protein 0206 medical engineering Autoimmunity 02 engineering and technology Biology Real-Time Polymerase Chain Reaction law.invention Young Adult 03 medical and health sciences 0302 clinical medicine law medicine Humans GENES DE RESPOSTA IMUNE RNA Messenger FADD Receptors Cytokine Promoter Regions Genetic General Dentistry Gene Polymerase chain reaction Aged Inflammation Periodontitis Interleukin-12 Subunit p40 Interleukin-4 Receptor alpha Subunit Promoter 030206 dentistry Methylation DNA Methylation Middle Aged medicine.disease 020601 biomedical engineering Molecular biology Gene Expression Regulation CpG site DNA methylation biology.protein Cytokines Chemokines Transcriptome Chemokines CXC Brazil Periapical Periodontitis |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12966 |
| Popis: | AIM To investigate the DNA methylation profiles of immune response-related genes in apical periodontitis (AP) lesions. METHODOLOGY The methylation profiles on the cytosine-phosphate-guanine (CpG) regions of 22 gene promoters involved in inflammation and autoimmunity were assessed in 60 human AP lesions and 24 healthy periodontal ligaments (controls) using a pathway-specific real-time polymerase chain reaction array (EpiTect® Methyl Signature PCR Array Human Inflammatory Response). Differentially methylated genes were subsequently assessed for their mRNA expression. Data analyses (One-way anova, Tukey's multiple comparisons tests and Mann-Whitney tests) were performed using GraphPad Prism 6 software. P values ≤ 0.05 were considered statistically significant. RESULTS Significant DNA hypermethylation was observed for CXCL3 and FADD gene promoters in AP lesions when compared to control tissues (P |
| Databáze: | OpenAIRE |
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