Hepatocyte Isolation and Transplantation in the Pig
| Autor: | Noriaki Tanaka, Michihiko Takesue, Yuzuru Kurabayashi, Naoya Kobayashi, Toshinori Totsugawa, Takemi Kunieda, Makoto Kodama, Mizuko Oshita, Shuhei Nakaji, Teru Okitsu, Norikuni Shibata, Masanobu Maruyama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology medicine.medical_specialty Cell Survival Cell Transplantation Sus scrofa Transplantation Heterologous 030232 urology & nephrology Biomedical Engineering lcsh:Medicine Galactosamine Cell Separation Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endopeptidases Dispase medicine Retrograde perfusion Animals Collagenases Survival rate Transplantation Liver Diseases lcsh:R Liver failure Cell Biology Liver Failure Acute Liver Transplantation Perfusion Survival Rate Disease Models Animal Treatment Outcome medicine.anatomical_structure chemistry Hepatocyte Hepatocytes Collagenase 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Cell Transplantation, Vol 12 (2003) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | Hepatocyte transplantation (HTX) has received great expectation for the treatment of a wide spectrum of liver diseases. Considering the severe shortage of human livers for hepatocyte isolation, porcine hepatocytes are an attractive alternative to normal human hepatocytes. To develop such therapy, establishment of an efficient hepatocyte isolation and transplantation model that enables accurate assessment of safety and efficacy of HTX is extremely important. Porcine hepatocytes were isolated from a surgically removed liver segment with a four-step retrograde perfusion using dispase and collagenase. The resultant hepatocytes of > 84% viability were used for transplantation experiment in a pig model of acute liver failure induced by intravenous administration of D-galactosamine (D-gal) (0.5 mg/kg). Twenty-four hours after D-gal injection, transplantation of freshly isolated porcine hepatocytes (1 x 10(9)) was safely conducted and prolonged the survival of D-gal-treated pigs. We describe an efficient porcine hepatocyte isolation and subsequent cell transplantation in pigs with D-gal-induced liver failure. |
| Databáze: | OpenAIRE |
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