CD36 family members are TCR-independent ligands for CD1 antigen-presenting molecules
| Autor: | Matthew E. Ritchie, Samuel J. Redmond, Adam P Uldrich, Catriona V. Nguyen-Robertson, Katherine H. A. Gourley, Catarina F. Almeida, D. Branch Moody, Hamish E G McWilliam, Shihan Li, Dale I. Godfrey, Nicholas A Gherardin, Fiona Ross, Daniel G. Pellicci, Robert De Rose, Rebecca Seneviratna, Jose A Villadangos, Shian Su |
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| Rok vydání: | 2021 |
| Předmět: |
CD36 Antigens
T cell Immunology Primary Cell Culture CD1 Receptors Antigen T-Cell chemical and pharmacologic phenomena Ligands Article Antigens CD1 03 medical and health sciences Jurkat Cells 0302 clinical medicine Antigen Tetramer T-Lymphocyte Subsets parasitic diseases medicine Humans Receptor 030304 developmental biology Glycoproteins 0303 health sciences Antigen Presentation biology Chemistry T-cell receptor hemic and immune systems General Medicine Natural killer T cell Lipids Healthy Volunteers 3. Good health Cell biology medicine.anatomical_structure CD1D Blood Buffy Coat biology.protein Protein Multimerization 030215 immunology |
| Zdroj: | Sci Immunol |
| ISSN: | 2470-9468 |
| Popis: | CD1c presents lipid-based antigens to CD1c-restricted T cells, which are thought to be a major component of the human T cell pool. However, the study of CD1c-restricted T cells is hampered by the presence of an abundantly expressed, non-T cell receptor (TCR) ligand for CD1c on blood cells, confounding analysis of TCR-mediated CD1c tetramer staining. Here, we identified the CD36 family (CD36, SR-B1, and LIMP-2) as ligands for CD1c, CD1b, and CD1d proteins and showed that CD36 is the receptor responsible for non-TCR-mediated CD1c tetramer staining of blood cells. Moreover, CD36 blockade clarified tetramer-based identification of CD1c-restricted T cells and improved identification of CD1b- and CD1d-restricted T cells. We used this technique to characterize CD1c-restricted T cells ex vivo and showed diverse phenotypic features, TCR repertoire, and antigen-specific subsets. Accordingly, this work will enable further studies into the biology of CD1 and human CD1-restricted T cells. |
| Databáze: | OpenAIRE |
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