A unified model for microtubule rescue
| Autor: | Colby P. Fees, Jeffrey K. Moore |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Cations
Divalent Biology Microtubules Polymerization Divalent 03 medical and health sciences 0302 clinical medicine Tubulin Microtubule Animals Humans Computer Simulation Molecular Biology Cytoskeleton 030304 developmental biology Stable state chemistry.chemical_classification 0303 health sciences Extramural Depolymerization Articles Cell Biology Unified Model Microtubule rescue Actin Depolymerizing Factors chemistry Biophysics biology.protein Monte Carlo Method 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology of the Cell |
| DOI: | 10.1101/401372 |
| Popis: | How microtubules transition from depolymerization to polymerization, known as rescue, is poorly understood. Here we examine two models for rescue: 1) an ‘end-driven’ model in which the depolymerizing end stochastically switches to a stable state; and 2) a ‘lattice-driven’ model in which rescue-sites are integrated into the microtubule prior to depolymerization. We test these models using a combination of computational simulations andin vitroexperiments with purified tubulin. Our findings support the ‘lattice-driven’ model by identifying repeated rescue sites in microtubules. In addition, we discover an important role for divalent cations in determining the frequency and location of rescue sites. We use ‘wash-in’ experiments to show that divalent cations inhibit rescue during depolymerization, but not during the polymerization. We propose a unified model in which rescues are driven by embedded rescue sites in microtubules, but the activity of these sites is influenced by changes in the depolymerizing ends. |
| Databáze: | OpenAIRE |
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