Mitochondrial formation in the developing flight muscles of the Colorado beetle
| Autor: | A.M. Kroon, A.K.M. Bartelink, W.P. Gijzel, C.A.D. de Kort |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Glycerolphosphate Dehydrogenase
Mitochondrion Biology Cycloheximide Electron Transport Complex IV chemistry.chemical_compound Oxygen Consumption Leucine Ethidium Animals Cytochrome c oxidase Carbon Radioisotopes Molecular Biology chemistry.chemical_classification Muscles Succinate dehydrogenase Cell Biology Mitochondria Muscle Coleoptera Succinate Dehydrogenase Enzyme chemistry Biochemistry Flight Animal Protein Biosynthesis biology.protein Cytochromes Ethidium bromide Developmental Biology |
| Zdroj: | Developmental Biology. 42:262-273 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(75)90333-4 |
| Popis: | Flight-muscle development in the Colorado beetle ( Leptinotarsa decemlineata Say) is accompanied by rapid accumulation of mitochondrial enzymes. The elaboration of succinate dehydrogenase and cytochrome c oxidase activities and the contents of cytochromes aa 3 , b and c was found to proceed almost in parallel. Ethidium bromide, an inhibitor of mitochondrial transcription, inhibited the increase in cytochrome c oxidase activity, indicating that the intrinsic genetic system of the flight-muscle mitochondria contributes to the formation of the enzyme. The role of the mitochondrial protein-synthesizing system during development was studied more specifically with pulse-labelling experiments both in vivo and in vitro. In vivo , the incorporation of 14 C-leucine into mitochondrial protein was measured in the presence and absence of the cytoplasmic ribosomal inhibitor cycloheximide. At the beginning of flight-muscle development and in full-grown muscles, about 5% of amino acid incorporation was resistant to the action of the antibiotic. During the period of rapid accumulation of respiratory enzymes, the percentage of cycloheximide-resistant incorporation rose to 14. Also flight-muscle mitochondria isolated at this stage showed the highest 14 C-leucine incorporation in vitro . Evidently, mitochondrial formation is associated with a higher contribution of the intrinsic protein-synthesizing system. |
| Databáze: | OpenAIRE |
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