Induction of Chicken Host Defense Peptides within Disease-Resistant and -Susceptible Lines
Autor: | Melissa S. Monson, Michael G. Kaiser, Susan J. Lamont, Hyun-Jun Jang |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Lipopolysaccharides Cell type animal structures lcsh:QH426-470 Lipopolysaccharide chicken Bone Marrow Cells Biology Article Avian Proteins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Downregulation and upregulation GNLY Gene expression Genetics Animals Gene Genetics (clinical) Poultry Diseases Disease Resistance host defense peptides Gene Expression Profiling Fibroblasts Molecular biology Embryonic stem cell immune responses lcsh:Genetics 030104 developmental biology chemistry Gene Expression Regulation 030220 oncology & carcinogenesis embryonic structures gene expression Disease Susceptibility Chickens Antimicrobial Cationic Peptides |
Zdroj: | Genes Volume 11 Issue 10 Genes, Vol 11, Iss 1195, p 1195 (2020) |
ISSN: | 2073-4425 |
Popis: | Host defense peptides (HDPs) are multifunctional immune molecules that respond to bacterial and viral pathogens. In the present study, bone marrow-derived cells (BMCs) and chicken embryonic fibroblasts (CEFs) were cultured from a Leghorn line (Ghs6) and Fayoumi line (M15.2), which are inbred chicken lines relatively susceptible and resistant to various diseases, respectively. The cells were treated by lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (poly(I:C)) and, subsequently, mRNA expression of 20 chicken HDPs was analyzed before and after the stimulation. At homeostasis, many genes differed between the chicken lines, with the Fayoumi line having significantly higher expression (p < 0.05) than the Leghorn line: AvBD1, 2, 3, 4, 6, and 7 in BMCs CATH1, CATH3, and GNLY in CEFs and AvDB5, 8, 9, 10, 11, 12, 13 in both BMCs and CEFs. After LPS treatment, the expression of AvBD1, 2, 3, 4, 5, 9, 12, CATH1, and CATHB1 was significantly upregulated in BMCs, but no genes changed expression in CEFs. After poly(I:C) treatment, AvBD2, 11, 12, 13, CATHB1 and LEAP2 increased in both cell types CATH2 only increased in BMCs and AvBD3, 6, 9, 14, CATH1, CATH3, and GNLY only increased in CEFs. In addition, AvBD7, AvBD14, CATH1, CATH2, GNLY, and LEAP2 showed line-specific expression dependent upon cell type (BMC and CEF) and stimulant (LPS and poly(I:C)). The characterization of mRNA expression patterns of chicken HDPs in the present study suggests that their functions may be associated with multiple types of disease resistance in chickens. |
Databáze: | OpenAIRE |
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