Isolation and characterization of exosomes released from mosquito cells infected with dengue virus
| Autor: | Luis Adrián De Jesús-González, O.E. Cigarroa-Mayorga, Margot Cervantes-Salazar, Bibiana Chávez-Munguía, Rosa M. del Angel, Juan Fidel Osuna-Ramos, Arianna Mahely Hurtado-Monzón, José Manuel Reyes-Ruiz, Juan Santiago Salas-Benito, Eduardo San Martín-Martínez, Carlos Noe Farfan-Morales, Fernando Medina, Rogelio Fragoso-Soriano, Jeni Bolaños |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Aedes albopictus Tetraspanins viruses Mosquito Vectors Dengue virus medicine.disease_cause Exosomes Virus Replication Arbovirus Virus Cell Line Dengue 03 medical and health sciences Tetraspanin Microscopy Electron Transmission Aedes Virology medicine Animals Humans Phylogeny 030304 developmental biology 0303 health sciences Microscopy Confocal biology 030306 microbiology virus diseases Dengue Virus biology.organism_classification medicine.disease Microvesicles Dynamic Light Scattering Infectious Diseases biology.protein Insect Proteins Antibody |
| Zdroj: | Virus research. 266 |
| ISSN: | 1872-7492 |
| Popis: | Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naive C6/36 cells, suggesting that exosomes are playing a role in virus dissemination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect