A comparative analysis of derivatization strategies for the determination of biogenic amines in sausage and cheese by HPLC
| Autor: | Si-Jin Liu, Chunfeng Guo, Changlu Ma, Juan-Juan Xu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Biogenic Amines
Benzoates 01 natural sciences High-performance liquid chromatography Chloride Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Benzoyl chloride Cheese Biogenic amine medicine Derivatization Chromatography High Pressure Liquid Dansyl Compounds chemistry.chemical_classification Chromatography Solid Phase Extraction 010401 analytical chemistry Dansyl chloride 04 agricultural and veterinary sciences General Medicine Tyramine 040401 food science 0104 chemical sciences Meat Products chemistry Linear range Spectrophotometry Ultraviolet Food Analysis Food Science medicine.drug |
| Zdroj: | Food Chemistry. 266:275-283 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2018.06.001 |
| Popis: | The six biogenic amines in sausage and cheese were analyzed by HPLC with UV detection after off-line derivatization with dansyl chloride, 9-fluorenylmethoxycarbonyl chloride, benzoyl chloride and dabsyl chloride, respectively. The results showed that both the off-line 9-fluorenylmethoxycarbonyl and dabsyl derivatization were not suitable for HPLC analysis of biogenic amines when batch injection was used because the derivatives were instable, whereas both the off-line dansyl and benzoyl derivatization were suitable for HPLC analysis of biogenic amines when batch injection was used, but the latter needed to maintain the derivatives at 4 °C to ensure that benzoylated tyramine was not degraded when waiting for the analysis. The off-line dansyl derivatization had an obvious advantage in the analysis of biogenic amines in sausage and cheese samples by HPLC combined with batch injection because the method has a wider linear range and higher sensitivity, accuracy, precision and stability of the derivatives. |
| Databáze: | OpenAIRE |
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