Intestinal microbiota composition in free-range pigs is associated with the presence of Salmonella
| Autor: | Lorenzo Fraile, Victoria Garrido, Inés Gaitán, Lourdes Migura-Garcia, Ilargi Martinez-Ballesteros, María Jesús Grilló, Ainhoa Arrieta-Gisasola |
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| Přispěvatelé: | Producció Animal, Sanitat Animal, Fundación Caja Navarra |
| Rok vydání: | 2021 |
| Předmět: |
Salmonella
Veterinary medicine Health (social science) Risk factors in diseases TP1-1185 Plant Science Gut flora medicine.disease_cause Health Professions (miscellaneous) Microbiology Article 03 medical and health sciences Clostridium Salmonel·la Lactobacillus microbiota medicine risk factors antimicrobial resistance Bacteroidaceae 030304 developmental biology 0303 health sciences biology 030306 microbiology Factors de risc en les malalties Chemical technology Microbiota Enterobacter biology.organism_classification free-range pigs Porcs Bacteroides Proteobacteria Food Science |
| Zdroj: | Repositorio Abierto de la UdL Universitad de Lleida Foods Addi. Archivo Digital para la Docencia y la Investigación instname Volume 10 Issue 6 Foods, Vol 10, Iss 1410, p 1410 (2021) Digital.CSIC. Repositorio Institucional del CSIC Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona IRTA Pubpro. Open Digital Archive Institut de Recerca i Tecnologia Agroalimentàries (IRTA) Addi: Archivo Digital para la Docencia y la Investigación Universidad del País Vasco |
| DOI: | 10.3390/foods10061410 |
| Popis: | Extensive pig systems are gaining importance as quality production systems and as the standard for sustainable rural development and animal welfare. However, the effects of natural foods on Salmonella epidemiology remain unknown. Herein, we assessed the presence of Salmonella and the composition of the gut microbiota in pigs from both Salmonella-free and high Salmonella prevalence farms. In addition, risk factors associated with the presence of Salmonella were investigated. The pathogen was found in 32.2% of animals and 83.3% of farms, showing large differences in prevalence between farms. Most isolates were serovars Typhimurium monophasic (79.3%) and Bovismorbificans (10.3%), and exhibited a multi-drug resistance profile (58.6%). Risk factor analysis identified feed composition, type/variety of vegetation available, and silos’ cleaning/disinfection as the main factors associated with Salmonella prevalence. Clear differences in the intestinal microbiota were found between Salmonella-positive and Salmonella-negative populations, showing the former with increasing Proteobacteria and decreasing Bacteroides populations. Butyrate and propionate producers including Clostridium, Turicibacter, Bacteroidaceae_uc, and Lactobacillus were more abundant in the Salmonella-negative group, whereas acetate producers like Sporobacter, Escherichia or Enterobacter were more abundant in the Salmonella-positive group. Overall, our results suggest that the presence of Salmonella in free-range pigs is directly related to the natural vegetation accessible, determining the composition of the intestinal microbiota. This work was funded by the Caja Navarra Foundation (project reference 70628). |
| Databáze: | OpenAIRE |
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