Penile Cancer-Derived Cells Molecularly Characterized as Models to Guide Targeted Therapies
| Autor: | Fabio Albuquerque Marchi, Eliney Ferreira Faria, Ademar Lopes, Luisa Matos do Canto, Mads M. Aagaard, Hellen Kuasne, Silvia Regina Rogatto, Cristovam Scapulatempo-Neto, Sébastien Carréno, Camille Valérie De Jamblinne, Juan José Augusto Moyano Muñoz |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell medicine.disease_cause 0302 clinical medicine Cell Movement Molecular Targeted Therapy lcsh:QH301-705.5 EGFR inhibitors Aged 80 and over Cetuximab CAFs General Medicine Middle Aged Neoplasm Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure 030220 oncology & carcinogenesis Erlotinib Signal Transduction medicine.drug Adult translatomic profile Biology Models Biological Article 03 medical and health sciences Gefitinib Cell Line Tumor medicine Humans Penile cancer Neoplasm Invasiveness RNA Messenger Cell Shape Penile Neoplasms protein expression PI3K/AKT/mTOR pathway genomic profile Aged Cell Proliferation cancer cell models Genome Human Gene Expression Profiling medicine.disease penile cancer 030104 developmental biology lcsh:Biology (General) Protein Biosynthesis Cancer research Carcinogenesis |
| Zdroj: | Cells Volume 10 Issue 4 Kuasne, H, Canto, L M D, Aagaard, M M, Muñoz, J J M, Jamblinne, C D, Marchi, F A, Scapulatempo-Neto, C, Faria, E F, Lopes, A, Carréno, S & Rogatto, S R 2021, ' Penile Cancer-Derived Cells Molecularly Characterized as Models to Guide Targeted Therapies ', Cells, vol. 10, no. 4, 814 . https://doi.org/10.3390/cells10040814 Cells, Vol 10, Iss 814, p 814 (2021) |
| ISSN: | 2073-4409 |
| DOI: | 10.3390/cells10040814 |
| Popis: | Penile cancer (PeCa) is a common disease in poor and developing countries, showing high morbidity rates. Despite the recent progress in understanding the molecular events involved in PeCa, the lack of well-characterized in vitro models precludes new advances in anticancer drug development. Here we describe the establishment of five human primary penile cancer-derived cell cultures, including two epithelial and three cancer-associated fibroblast (CAF) cells. Using high-throughput genomic approaches, we found that the epithelial PeCa derived- cells recapitulate the molecular alterations of their primary tumors and present the same deregulated signaling pathways. The differentially expressed genes and proteins identified are components of key oncogenic pathways, including EGFR and PI3K/AKT/mTOR. We showed that epithelial PeCa derived cells presented a good response to cisplatin, a common therapeutic approach used in PeCa patients. The growth of a PeCa-derived cell overexpressing EGFR was inhibited by EGFR inhibitors (cetuximab, gefitinib, and erlotinib). We also identified CAF signature markers in three PeCa-derived cells with fibroblast-like morphology, indicating that those cells are suitable models for PeCa microenvironment studies. We thus demonstrate the utility of PeCa cell models to dissect mechanisms that promote penile carcinogenesis, which are useful models to evaluate therapeutic approaches for the disease. |
| Databáze: | OpenAIRE |
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