Resveratrol and quercetin down-regulate tissue factor expression by human stimulated vascular cells
| Autor: | Roberto Lorenzet, R. Di Tommaso, A. Di Santo, Andrea Mezzetti, E. Napoleone, G. de Gaetano, Maria Benedetta Donati |
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| Rok vydání: | 2003 |
| Předmět: |
Umbilical Veins
Endothelium Active Transport Cell Nucleus Down-Regulation Wine Pharmacology Resveratrol Biology Monocytes Umbilical vein Thromboplastin Tissue factor chemistry.chemical_compound Western blot Stilbenes medicine Humans Dose-Response Relationship Drug medicine.diagnostic_test food and beverages Hematology Proto-Oncogene Proteins c-rel IκBα medicine.anatomical_structure Biochemistry chemistry I-kappa B Proteins Quercetin Tumor necrosis factor alpha Endothelium Vascular |
| Zdroj: | Journal of Thrombosis and Haemostasis. 1:1089-1095 |
| ISSN: | 1538-7836 |
| DOI: | 10.1046/j.1538-7836.2003.00217.x |
| Popis: | Summary. Background: Epidemiological studies have shown that consumption of wine reduces the risk of coronary heart disease. Resveratrol and quercetin, two polyphenolic compounds found in grapes and red wine, have been shown to contribute to this protection by exerting several biological properties which could be associated with cardioprotection. Tissue factor (TF), the cellular receptor that initiates blood coagulation, plays a primary role both in hemostasis following tissue injury and in the pathogenesis of atherosclerosis which predisposes to thrombosis. Objectives: We investigated the role of resveratrol and quercetin on TF expression by endothelial and mononuclear cells (MN). Methods: Confluent human umbilical vein endothelial cells and MN collected from healthy donors were stimulated with bacterial lipopolysaccharide, interleukin-1β or tumor necrosis factor-α after incubation with increasing concentrations of resveratrol or quercetin. Results: In both cell types, TF activity induced by any agonist was significantly reduced by resveratrol or quercetin in a dose-dependent fashion. Northern blot analysis indicated that resveratrol and quercetin strongly reduce TF mRNA in both cell types. The inhibition of TF mRNA originated from a reduction in nuclear binding activity of the transacting factor c-Rel/p65, which was induced by the agonists and measured by electromobility shift assay. Western blot analysis revealed that the diminished c-Rel/p65 activity was dependent upon inhibition of degradation of the c-Rel/p65 inhibitory protein IκBα. Conclusions: These results provide a molecular basis which could help explain the protective activity of red wine against cardiovascular disease. |
| Databáze: | OpenAIRE |
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