A ToF-SIMS study of the lateral organization of lipids and proteins in pulmonary surfactant systems
| Autor: | Frans J. Walther, Alan J. Waring, Eleonora Keating, Nils O. Petersen, Ruud A. W. Veldhuizen, Fred Possmayer |
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| Rok vydání: | 2011 |
| Předmět: |
Lipid Bilayers
protein lipid interaction Spectrometry Mass Secondary Ion Peptide Biochemistry lung surfactant Rats Sprague-Dawley Surface tension Atomic force microscopy Pulmonary surfactant Lipid bilayer Lung chemistry.chemical_classification 0303 health sciences Chemistry Air 030302 biochemistry & molecular biology Surfactant function protein function Lipids Lateral compression peptide Secondary ion mass spectrometry Cholesterol lipids (amino acids peptides and proteins) surface property Surface Properties Biophysics protein localization Article 03 medical and health sciences Monolayer Animals Surface Tension phospholipid 030304 developmental biology Time-of-flight secondary mass spectrometry Chromatography Proteins Pulmonary Surfactants Cell Biology Langmuir Blodgett film time of flight secondary mass spectrometry Lipids proteins lateral organization Rats Pulmonary Alveoli Cattle phase separation |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1808:614-621 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/j.bbamem.2010.11.015 |
| Popis: | Pulmonary surfactant is a complex lipid-protein mixture whose main function is to reduce the surface tension at the air-liquid interface of alveoli to minimize the work of breathing. The exact mechanism by which surfactant monolayers and multilayers are formed and how they lower surface tension to very low values during lateral compression remains uncertain. We used time-of-flight secondary ion mass spectrometry to study the lateral organization of lipids and peptide in surfactant preparations ranging in complexity. We show that we can successfully determine the location of phospholipids, cholesterol and a peptide in surfactant Langmuir-Blodgett films and we can determine the effect of cholesterol and peptide addition. A thorough understanding of the lateral organization of PS interfacial films will aid in our understanding of the role of each component as well as different lipid-lipid and lipid-protein interactions. This may further our understanding of pulmonary surfactant function. © 2010 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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