Evolution and function of the neisserial dam-replacing gene
| Autor: | Giuseppina Cantalupo, Cecilia Bucci, Caterina Pagliarulo, Paola Salvatore, Pietro Alifano, Carmelo B. Bruni, Vera Roberti, Alfredo Lavitola |
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| Přispěvatelé: | Cantalupo, G, Bucci, Cecilia, Salvatore, P., Pagliarulo, C., Roberti, V., Lavitola, A, Bruni, C. B., Alifano, Pietro, Bucci, C, Salvatore, Paola, Pagliarulo, C, Roberti, V, Lavitola, Alfredo, Bruni, CARMELO BRUNO, Alifano, P. |
| Rok vydání: | 2001 |
| Předmět: |
Molecular Sequence Data
Biophysics DNA repair Neisseria meningitidis medicine.disease_cause Biochemistry Microbiology Evolution Molecular Listeria monocytogenes Bacterial Proteins Structural Biology Sequence Homology Nucleic Acid Genetics medicine Neisseria meningitidi RNA Messenger Deoxyribonucleases Type II Site-Specific Molecular Biology Gene Neisseria lactamica Repetitive Sequences Nucleic Acid Phase variation Type II restriction endonuclease Non-pathogenic Neisseria biology Base Sequence NmeBII Lactococcus lactis Neisserial small repeated element Cell Biology biology.organism_classification Restriction enzyme Staphylococcus aureus Genes Bacterial |
| Zdroj: | FEBS letters. 495(3) |
| ISSN: | 0014-5793 |
| Popis: | Phase variation through slippage-like mechanisms involving homopolymeric tracts depends in part on the absence of Dam-methylase in several pathogenic isolates of Neisseria meningitidis. In Dam-defective strains drg (dam-replacing gene), flanked by pseudo-transposable small repeated elements (SREs), replaced dam. We demonstrate that drg encodes a restriction endonuclease (NmeBII) that cleaves 5′-GmeATC-3′. drg is also present in 50% of Neisseria lactamica strains, but in most of them it is inactive because of the absence of an SRE-providing promoter. This is associated with the presence of GATmeC, suggesting an alternative restriction-modification system (RM) specific for 5′-GATC-3′, similar to Sau3AI-RM of Staphylococcus aureus 3A, Lactococcus lactis KR2 and Listeria monocytogenes. |
| Databáze: | OpenAIRE |
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