Plasmonic gratings with nano-protrusions made by glancing angle deposition for single-molecule super-resolution imaging
| Autor: | Avinash Pathak, Steven C. Hamm, Haisheng Zheng, Keshab Gangopadhyay, Sagnik Basuray, Aaron Wood, Sangho Bok, Sheila A. Grant, Biyan Chen, Joseph Mathai, Shubhra Gangopadhyay, Peter V. Cornish |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Fluorophore Nanotechnology 02 engineering and technology Substrate (electronics) Grating 021001 nanoscience & nanotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Physical vapor deposition Nano Microscopy General Materials Science Surface plasmon resonance 0210 nano-technology Plasmon |
| Zdroj: | Nanoscale. 8(24) |
| ISSN: | 2040-3372 |
| Popis: | Super-resolution imaging has been advantageous in studying biological and chemical systems, but the required equipment and platforms are expensive and unable to observe single-molecules at the high (μM) fluorophore concentrations required to study protein interaction and enzymatic activity. Here, a plasmonic platform was designed that utilized an inexpensively fabricated plasmonic grating in combination with a scalable glancing angle deposition (GLAD) technique using physical vapor deposition. The GLAD creates an abundance of plasmonic nano-protrusion probes that combine the surface plasmon resonance (SPR) from the periodic gratings with the localized SPR of these nano-protrusions. The resulting platform enables simultaneous imaging of a large area without point-by-point scanning or bulk averaging for the detection of single Cyanine-5 molecules in dye concentrations ranging from 50 pM to 10 μM using epifluorescence microscopy. Combining the near-field plasmonic nano-protrusion probes and super-resolution technique using localization microscopy, we demonstrate the ability to resolve grain sizes down to 65 nm. This plasmonic GLAD grating is a cost-effective super-resolution imaging substrate with potential applications in high-speed biomedical imaging over a wide range of fluorescent concentrations. |
| Databáze: | OpenAIRE |
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