Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst
| Autor: | Hao Zhang, Zu Hangtian, Shujun Wang, Dongxue Dong, Ding Yanshuai, Cang Wang, Mingsheng Lyu, Wang Xuelian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Immobilized enzyme
Sodium chemistry.chemical_element 02 engineering and technology lcsh:Technology Hydrolysate Article recyclable 03 medical and health sciences chemistry.chemical_compound Hydrolysis Desorption Sodium fluoride Sodium citrate General Materials Science lcsh:Microscopy lcsh:QC120-168.85 030304 developmental biology 0303 health sciences Dextranase lcsh:QH201-278.5 lcsh:T dextranase hydroxyapatite 021001 nanoscience & nanotechnology chemistry lcsh:TA1-2040 immobilization lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology plaque removal lcsh:TK1-9971 Nuclear chemistry |
| Zdroj: | Materials Volume 14 Issue 1 Materials, Vol 14, Iss 130, p 130 (2021) |
| ISSN: | 1996-1944 |
| Popis: | The immobilization technology provides a potential pathway for enzyme recycling. Here, we evaluated the potential of using dextranase immobilized onto hydroxyapatite nanoparticles as a promising inorganic material. The optimal immobilization temperature, reaction time, and pH were determined to be 25 ° C, 120 min, and pH 5, respectively. Dextranase could be loaded at 359.7 U/g. The immobilized dextranase was characterized by field emission gun-scanning electron microscope (FEG-SEM), X-ray diffraction (XRD), and Fourier-transformed infrared spectroscopy (FT-IR). The hydrolysis capacity of the immobilized enzyme was maintained at 71% at the 30th time of use. According to the constant temperature acceleration experiment, it was estimated that the immobilized dextranase could be stored for 99 days at 20 ° C, indicating that the immobilized enzyme had good storage properties. Sodium chloride and sodium acetic did not desorb the immobilized dextranase. In contrast, dextranase was desorbed by sodium fluoride and sodium citrate. The hydrolysates were 79% oligosaccharides. The immobilized dextranase could significantly and thoroughly remove the dental plaque biofilm. Thus, immobilized dextranase has broad potential application in diverse fields in the future. |
| Databáze: | OpenAIRE |
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