Effect of Gloriosa superba linn (EEGS) on mPT and monosodium glutamate-induced proliferative disorder using rat model
| Autor: | Adeola Oluwakemi Olowofolahan, Olufunso O. Olorunsogo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Prostatic Diseases Monosodium glutamate Uterine hyperplasia Apoptosis Mitochondria Liver Pharmacology Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Discovery Sodium Glutamate medicine Animals Rats Wistar Caspase 030304 developmental biology Cell Proliferation Membrane Potential Mitochondrial Uterine Diseases 0303 health sciences Hyperplasia biology Plant Extracts Cytochrome c Uterus Prostate medicine.disease Disease Models Animal Mitochondrial permeability transition pore chemistry Liver 030220 oncology & carcinogenesis biology.protein Female Lipid Peroxidation medicine.symptom Chemical and Drug Induced Liver Injury Colchicaceae Apoptosis Regulatory Proteins Signal Transduction |
| Zdroj: | Journal of ethnopharmacology. 267 |
| ISSN: | 1872-7573 |
| Popis: | Ethnopharmacological relevance Hyperplasia, Tumors and cancers are various forms of proliferative disorders affecting humans. Surgery is the main treatment approach while other options are also associated with adverse effects. There is therefore a need for the development of better alternative therapy that is cost effective and readily available with little or no adverse effect. Some bioactive agents in medicinal plants exhibit their anti-proliferative potential by induction of mitochondrial permeability transition pore (mPT) opening. Gloriosa superba, a medicinal plant, is folklorically used in the treatment of tumors and cancers. Aim of the study: This study therefore aimed at investigating the effect of ethanol leaf extract of Gloriosa superba (EEGS) on mPT and monosodium glutamate-induced proliferative disorder in some specific tissues using rat model. Materials and methods Isolated rat liver mitochondria were exposed to different concentrations (10, 30, 50, 70 and 90 μg/ml) of EEGS. The mPT pore opening, cytochrome c release, mitochondrial ATPase activity and lipid peroxidation were assessed spectrophotometrically. Caspases 9 and 3 activities were carried out using ELISA technique. Histological assessment of the liver, prostate and uterus of normal and monosodium glutamate (MSG)-treated rats were carried out. Results The results showed significant induction of mPT pore opening, release of cytochrome c, enhancement of mitochondrial ATPase activity, inhibition of lipid peroxidation and activation of caspases 9 and 3 activities by EEGS. The histological assessment revealed the presence of MSG-induced hepato-cellular damage, benign prostate hyperplasia and uterine hyperplasia which were ameliorated by EEGS co-administration. Conclusions These findings suggest that EEGS contains putative agents that can induce apoptosis via induction of mPT pore opening and as well protect against MSG-induced hepato-cellular damage and proliferative disorder in prostate and uterus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect