M cell-targeted DNA vaccination
| Autor: | Xinhai Wang, Keri L. Csencsits, Asmahan Haddad, David W. Pascual, Yunpeng Wu, Nancy Walters |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Mice
Inbred BALB C Multidisciplinary Transgene Gene Transfer Techniques Enzyme-Linked Immunosorbent Assay Biology Biological Sciences Viral Nonstructural Proteins Virology DNA vaccination Vaccination chemistry.chemical_compound Mice chemistry Immunity Polylysine Immunology Vaccines DNA Cytotoxic T cell Respiratory epithelium Animals Microfold cell T-Lymphocytes Cytotoxic |
| Popis: | DNA immunization, although attractive, is poor for inducing mucosal immunity, thus limiting its protective value against most infectious agents. To surmount this shortcoming, we devised a method for mucosal transgene vaccination by using an M cell ligand to direct the DNA vaccine to mucosal inductive tissues and the respiratory epithelium. This ligand, reovirus protein final sigma1, when conjugated to polylysine (PL), can bind the apical surface of M cells from nasal-associated lymphoid tissues. Intranasal immunizations with protein final sigma1-PL-DNA complexes produced antigen-specific serum IgG and prolonged mucosal IgA, as well as enhanced cell-mediated immunity, made evident by elevated pulmonary cytotoxic T lymphocyte responses. Therefore, targeted transgene vaccination represents an approach for enabling DNA vaccination of the mucosa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|