Cryptochrome 2 and phototropin 2 regulate resistance protein-mediated viral defense by negatively regulating an E3 ubiquitin ligase
| Autor: | Daniel F. Klessig, Rae-Dong Jeong, Aardra Kachroo, Pradeep Kachroo, Subhankar Roy Barman, A. C. Chandra-Shekara, Duroy A. Navarre |
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| Rok vydání: | 2010 |
| Předmět: |
animal structures
Phototropin Light genetic structures Ubiquitin-Protein Ligases Green Fluorescent Proteins Immunoblotting Arabidopsis Repressor chemistry.chemical_compound Cryptochrome Gene Expression Regulation Plant MG132 medicine Psychological repression Plant Diseases Microscopy Confocal Multidisciplinary biology Arabidopsis Proteins Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Turnip crinkle virus Biological Sciences Plants Genetically Modified biology.organism_classification Molecular biology Immunity Innate eye diseases Ubiquitin ligase Cell biology Cryptochromes Plant Leaves Repressor Proteins chemistry Host-Pathogen Interactions Mutation Proteasome inhibitor biology.protein Carmovirus sense organs Salicylic Acid Protein Binding Signal Transduction medicine.drug |
| Zdroj: | Proceedings of the National Academy of Sciences. 107:13538-13543 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Light harvested by plants is essential for the survival of most life forms. This light perception ability requires the activities of proteins termed photoreceptors. We report a function for photoreceptors in mediating resistance (R) protein-derived plant defense. The blue-light photoreceptors, cryptochrome (CRY) 2 and phototropin (PHOT) 2, are required for the stability of the R protein HRT, and thereby resistance to Turnip Crinkle virus (TCV). Exposure to darkness or blue-light induces degradation of CRY2, and in turn HRT, resulting in susceptibility. Overexpression of HRT can compensate for the absence of PHOT2 but not CRY2. HRT does not directly associate with either CRY2 or PHOT2 but does bind the CRY2-/PHOT2-interacting E3 ubiquitin ligase, COP1. Application of the proteasome inhibitor, MG132, prevents blue-light-dependent degradation of HRT, consequently these plants show resistance to TCV under blue-light. We propose that CRY2/PHOT2 negatively regulate the proteasome-mediated degradation of HRT, likely via COP1, and blue-light relieves this repression resulting in HRT degradation. |
| Databáze: | OpenAIRE |
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