Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
| Autor: | Azadeh Montaseri, Mehdi Hassanpour, Soheila Montazersaheb, Alireza Garjani, Hassan Malekinejad, Elahe Nabat, Reza Rahbarghazi, Aysa Rezabakhsh, Ali Akbar Movassaghpour |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Medicine (General) QH301-705.5 Angiogenesis Endothelial cells Pharmaceutical Science High glucose condition Pharmacology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Nitric oxide 03 medical and health sciences chemistry.chemical_compound R5-920 0302 clinical medicine medicine TBARS MTT assay Viability assay Biology (General) Original Research chemistry.chemical_classification Reactive oxygen species General Medicine Tubulogenesis Malondialdehyde endothelial cells high glucose condition hydroxychloroquine oxidative stress tubulogenesis 030104 developmental biology chemistry Oxidative stress 030220 oncology & carcinogenesis Hydroxychloroquine |
| Zdroj: | BioImpacts : BI BioImpacts, Vol 7, Iss 4, Pp 219-226 (2017) |
| ISSN: | 2228-5652 2228-5660 |
| DOI: | 10.15171/bi.2017.26 |
| Popis: | Introduction: Under the diabetic condition, sustained production of oxidative/nitrosative stress results in irreversible vascular injuries. A great number of diabetic pathologies, such as inefficient or aberrant neo-angiogenesis emerge following chronic hyperglycemic condition. Lack of enough data exists regarding hydroxychloroquine (HCQ) contribution on angiogenesis during diabetes mellitus. Methods: To better address whether HCQ could blunt or exacerbate oxidative status and angiogenesis under high glucose condition (HCG), human umbilical vein endothelial cells (HUVECs) were exposed to 30 µM HCQ in combination with 30 mM glucose over a course of 72 hours. Viability was measured was evaluated by MTT assay. We used Griess method and TBARS assay to monitor changes in the levels of NO and MDA followed by flow cytometric analysis of ROS using DCFDA. To show the impact of HCQ on cell motility and in vitro angiogenic properties, we exploited routine scratch test and in vitro tubulogenesis, respectively. Results: Our data showed that HCQ diminished cell viability under 5 and 30 mM glucose contents. HCQ significantly decreased the total levels of nitric oxide (NO), malondialdehyde (MDA), and reactive oxygen species (ROS) in both sets of environments. Additionally, inhibitory effects were observed on cell migration after exposure to HCQ (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|