Th1/Th2 Immune Response in Lung Fibroblasts in Interstitial Lung Disease
| Autor: | Yoshinori Hasegawa, Kenzo Takagi, Masakazu Okamoto, Kazuyoshi Imaizumi, Kaoru Shimokata, Atsushi Sumida, Hiroshi Yatsuya, Tsutomu Kawabe, Toyoharu Yokoi, Naozumi Hashimoto |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Biology Th2 Cells Humans CXCL10 CCL17 CXCL11 RNA Messenger CCL13 Cells Cultured Aged CCL18 General Medicine Fibroblasts Middle Aged Th1 Cells respiratory system respiratory tract diseases CCL20 CXCL2 Gene Expression Regulation Immunology Female CCL27 Chemokines Lung Diseases Interstitial Bronchoalveolar Lavage Fluid |
| Zdroj: | Archives of Medical Research. 39:503-510 |
| ISSN: | 0188-4409 |
| DOI: | 10.1016/j.arcmed.2008.02.005 |
| Popis: | Background Inflammatory response in pulmonary fibrosis closely resembles a T-helper (Th) 2 immune response. For recruitment to an inflammatory lesion, the majority of Th1 cells express CXC chemokine receptor 3, recognizing monokine induced by interferon-gamma (Mig), interferon γ-inducible protein of 10 kD (IP-10), and interferon-inducible T-cell α chemoattractant (I-TAC). Th2 cells express CC chemokine receptor 4, recognizing thymus- and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC). We investigated the Th1/Th2 chemokine production patterns by lung fibroblasts and their evaluation in bronchoalveolar lavage (BAL) fluid of interstitial lung disease. Methods The production pattern of Th1/Th2 chemokines by lung fibroblasts was examined in ELISA and quantitative reverse transcriptase polymerase chain reactions. Th1/Th2 chemokine levels in BAL fluid of idiopathic pulmonary fibrosis (IPF) and nonspecific interstitial pneumonia (NSIP) were examined to evaluate the clinical relevance of Th1/Th2 chemokines. Results The lung fibroblasts were polarized to produce Th1-type chemokines by the pro-inflammatory cytokine, tumor necrosis factor (TNF)-α and the anti-fibrotic cytokine, interferon (IFN)-γ. However, the induction patterns of chemokines by these two cytokines were different, i.e., involving predominant induction of IP-10 and I-TAC by TNF-α and induction of Mig by IFN-γ. Although Mig, IP-10, and I-TAC were produced within the BAL fluid of patients, TARC and MDC were at significantly low levels. Conclusions Our results suggest that lung fibroblasts tend to induce a Th1-type immune response under normal conditions, and that a Th2-type immune response does not play a significant role in smoldering inflammation around the established lesions in IPF and NSIP. |
| Databáze: | OpenAIRE |
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