Large-Scale Electron Microscopy Maps of Patient Skin and Mucosa Provide Insight into Pathogenesis of Blistering Diseases
| Autor: | Gilles F. H. Diercks, Ben N G Giepmans, Duco Kramer, Jeroen Kuipers, Hendri H. Pas, Marcel F. Jonkman, Ena Sokol |
|---|---|
| Přispěvatelé: | Translational Immunology Groningen (TRIGR), Microbes in Health and Disease (MHD), Center for Liver, Digestive and Metabolic Diseases (CLDM), Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE) |
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Dermatology Sensitivity and Specificity Biochemistry law.invention ULTRASTRUCTURE Blister PEMPHIGUS FOLIACEUS Reference Values law BINDING Microscopy medicine Humans skin and connective tissue diseases Molecular Biology Pemphigus foliaceus Skin VULGARIS HYPOTHESIS Skin Diseases Vesiculobullous integumentary system Chemistry Acantholysis KERATINOCYTES Biopsy Needle Resolution (electron density) Mouth Mucosa DESMOGLEIN 1 Desmosomes Cell Biology medicine.disease Immunohistochemistry Nanostructures BULLOUS IMPETIGO Microscopy Electron Pemphigus Desmoglein 1 Case-Control Studies Ultrastructure Electron microscope |
| Zdroj: | Journal of Investigative Dermatology, 135(7), 1763-1770. ELSEVIER SCIENCE INC |
| ISSN: | 0022-202X |
| Popis: | Large-scale electron microscopy ("nanotomy") allows straight forward ultrastructural examination of tissue, cells, organelles, and macromolecules in a single data set. Such data set equals thousands of conventional electron microscopy images and is freely accessible (www.nanotomy.org). The software allows zooming in and out of the image from total overview to nanometer scale resolution in a 'Google Earth' approach. We studied the life-threatening human autoimmune blistering disease pemphigus, using nanotomy. The pathomechanism of cell-cell separation (acantholysis) that underlies the blistering is poorly understood. Ultrastructural examination of pemphigus tissue revealed previously unreported findings: (i) the presence of double-membrane structures between cells in all pemphigus types; (ii) the absence of desmosomes around spontaneous blisters in pemphigus foliaceus (PF); (iii) lower level blistering in PF when force induced; and (iv) intercellular widening at non-acantholytic cell layers. Thus, nanotomy delivers open-source electron microscopic maps of patient tissue, which can be analyzed for additional anomalies from any computer by experts from different fields. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|