Au@Ag Core@Shell Nanoparticles Synthesized with Rumex hymenosepalus as Antimicrobial Agent

Autor: César Rodríguez-Beas, Alfonso García-Galaz, Roberto Mora-Monroy, Eduardo Larios-Rodríguez, Blanca Esthela Rodríguez-Vázquez, Ericka Rodríguez-León, Ramón Iñiguez-Palomares, Heriberto Acuña-Campa, Roberto Carlos Carillo-Torres, Aarón Martínez-Higuera, Francisco Javier Alvarez-Cirerol, Jesús Mauro Adolfo Villalobos-Noriega, Maribel Plascencia-Jatomea
Rok vydání: 2021
Předmět:
Zdroj: Nanoscale Research Letters
Nanoscale Research Letters, Vol 16, Iss 1, Pp 1-19 (2021)
ISSN: 1931-7573
Popis: In this work, we used a sequential method of synthesis for gold–silver bimetallic nanoparticles with core@shell structure (Au@AgNPs). Rumex hymenosepalus root extract (Rh), which presents high content in catechins and stilbenes, was used as reductor agent in nanoparticles synthesis. Size distribution obtained by Transmission Electron Microscopy (TEM) gives a mean diameter of 36 ± 11 nm for Au@AgNPs, 24 ± 4 nm for gold nanoparticles (AuNPs), and 13 ± 3 nm for silver nanoparticles (AgNPs). The geometrical shapes of NPs were principally quasi-spherical. The thickness of the silver shell over AuNPs is around 6 nm and covered by active biomolecules onto the surface. Nanoparticles characterization included high angle annular dark field images (HAADF) recorded with a scanning transmission electron microscope (STEM), Energy-Dispersive X-ray Spectroscopy (EDS), X-Ray Diffraction (XRD), UV–Vis Spectroscopy, Zeta Potential, and Dynamic Light Scattering (DLS). Fourier Transform Infrared Spectrometer (FTIR), and X-ray Photoelectron Spectroscopy (XPS) show that nanoparticles are stabilized by extract molecules. A growth kinetics study was performed using the Gompertz model for microorganisms exposed to nanomaterials. The results indicate that AgNPs and Au@AgNPs affect the lag phase and growth rate of Escherichia coli and Candida albicans in a dose-dependent manner, with a better response for Au@AgNPs
Databáze: OpenAIRE
Externí odkaz: