Melatonin protects against streptozotocin-induced diabetic cardiomyopathy through themammalian target of rapamycin (mTOR) signaling pathway
| Autor: | Unal Guntekin, Yasemin Behram Kandemir, Veysel Tosun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Cardiac function curve medicine.medical_specialty Diabetic Cardiomyopathies Cardiomyopathy Medicine (miscellaneous) Streptozocin General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Melatonin Internal medicine Diabetes mellitus Diabetic cardiomyopathy Internal Medicine medicine Animals Pharmacology (medical) Rats Wistar Genetics (clinical) PI3K/AKT/mTOR pathway chemistry.chemical_classification business.industry TOR Serine-Threonine Kinases Glutathione peroxidase medicine.disease Streptozotocin Rats Endocrinology chemistry Reviews and References (medical) business Signal Transduction medicine.drug |
| Zdroj: | Advances in Clinical and Experimental Medicine. 28:1171-1177 |
| ISSN: | 1899-5276 |
| Popis: | Background Several studies demonstrated that the overexpression of mammalian target of rapamycin (mTOR) signaling protein is associated with cardiomyopathy. However, the effect of mTOR on the heart in hyperglycemic condition is still controversial. Objectives We aimed to investigate the expression of mTOR and antioxidant enzyme activity in cardiac hypertrophy in rats with streptozotocin-induced diabetes mellitus (DM), and the effects of the melatonin on diabetic cardiomyopathy (DCM). Material and methods Forty male Wistar rats were used as the experimental animals. The rats were divided into 4 groups (10 animals in each): group 1 (control group), group 2 (ethanol vehicle group), group 3 (iatrogenically DM-induced group), and group 4 (group given melatonin after iatrogenical DM induction). Streptozotocin was injected intraperitoneally to group 3 and 4 to induce experimental type 1 DM. Melatonin was injected intraperitoneally at a dose of 50 mg/kg/day for 56 days to group 4. We investigated expression of mTOR levels in heart muscle fibers of all groups. Laboratory analysis and transthoracic echocardiography were performed. Results Melatonin increased the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), which were reduced due to hyperglycemia. The mTOR expression levels were significantly higher in group 3 (DM group) compared with controls, whereas melatonin treatment significantly decreased the levels of mTOR expression in group 4 (melatonin + DM group). Diabetic rats developed myocardial hypertrophy with preserved cardiac function. Conclusions Cardioprotective effect of melatonin may reduce damages caused by DM in the heart muscle fibers through the mTOR signaling pathway. |
| Databáze: | OpenAIRE |
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