Carcinogenesis in rats subjected to a new model ureterosigmoidostomy and treated with L-lysine
| Autor: | Conceição Aparecida Dornelas, Nayanna de Oliveira Ramos Melo, Francisco Vagnaldo Fechine Jamacaru, Camila de Carvalho Juanes, Rafael Barbosa de Azevedo, Joao Paulo Ferreira Coelho, Alessandra Marques Dos Santos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Urologic Neoplasms RD1-811 Carcinogenesis medicine.medical_treatment H&E stain Urinary Diversion Gastroenterology complex mixtures Urothelial Hyperplasia 03 medical and health sciences Ureterosigmoidostomy 0302 clinical medicine Aberrant Crypt Foci Colon Sigmoid Internal medicine Intestinal Neoplasms medicine Atypia Animals Intestinal Mucosa Rats Wistar Ureterostomy business.industry Lysine Surgical Stomas Hyperplasia medicine.disease Rats Disease Models Animal Urinary Bladder Neoplasms Dysplasia 030220 oncology & carcinogenesis Celecoxib bacteria 030211 gastroenterology & hepatology Female Surgery business medicine.drug Aberrant crypt foci |
| Zdroj: | Acta Cirurgica Brasileira, Vol 31, Iss 12, Pp 793-800 (2016) Acta Cirurgica Brasileira, Volume: 31, Issue: 12, Pages: 793-800, Published: DEC 2016 Acta Cirúrgica Brasileira v.31 n.12 2016 Acta Cirúrgica Brasileira Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
| ISSN: | 0102-8650 |
| Popis: | PURPOSE: To evaluate the effects of L-lysine on the intestinal and urothelial epithelium of rats subjected to ureterosigmoidostomy (new model for surgical carcinogenesis). METHODS: Forty-two rats, 9 weeks of age, were divided into 6 groups. Animals in groups A, B, C were subjected to ureterosigmoidostomy (US) and treated with L-lysine, celecoxib and H2O, respectively. Groups D, E and F (non-operated controls) received L-lysine, celecoxib and H2O, respectively. The L-lysine dose was 150 mg/kg and that of celecoxib was 20 mg/kg. The colon was analyzed for the presence of aberrant crypt foci (ACF) under a stereomicroscope.The tissue was stained with hematoxylin and eosin and PAS alcian blue. RESULTS: There were rare ACF, and there was no statistically significant difference between the groups. Histopathologic study of the ureteral epithelium identified moderate to severe urothelial hyperplasia in rats with ureterosigmoidostomy. Transitional hyperplasia in the ureters of animals receiving L-lysine (A) showed an apparent difference compared to the control (C) (P=0.2424). There was no dysplasia or atypia CONCLUSION: L-lysine does not promote carcinogenesis of the intestinal and urethelial epithelium of rats subjected to ureterosigmoidostomy at the doses and times studied. |
| Databáze: | OpenAIRE |
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