A positive correlation between atypical memory B cells and Plasmodium falciparum transmission intensity in cross-sectional studies in Peru and Mali

Autor: Jean N. Hernandez, Aissata Ongoiba, Eva H. Clark, Boubacar Traore, Susan K. Pierce, Kassoum Kayentao, Ogobara K. Doumbo, Greta E Weiss, Shanping Li, Peter D. Crompton, OraLee H. Branch
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Male
B Cells
Anatomy and Physiology
lcsh:Medicine
Adaptive Immunity
Protozoology
Mali
0302 clinical medicine
Recurrence
Immune Physiology
Peru
Malaria
Falciparum
Memory B cell
lcsh:Science
Immune Response
0303 health sciences
B-Lymphocytes
Multidisciplinary
Middle Aged
Flow Cytometry
3. Good health
Plasmodium Falciparum
Host-Pathogen Interaction
medicine.anatomical_structure
Medicine
Female
Antibody
Research Article
Adult
Immune Cells
Immunology
Context (language use)
Biology
Microbiology
CD19
Antibodies
Immunomodulation
03 medical and health sciences
Young Adult
Antigen
Antigens
CD
parasitic diseases
medicine
Humans
Lymphocyte Count
Immunity to Infections
B cell
030304 developmental biology
Aged
lcsh:R
Immunity
Plasmodium falciparum
biology.organism_classification
medicine.disease
Cross-Sectional Studies
Humoral Immunity
biology.protein
Parastic Protozoans
Parasitology
lcsh:Q
Immunologic Memory
Malaria
030215 immunology
Zdroj: PLoS ONE, Vol 6, Iss 1, p e15983 (2011)
PLoS ONE
ISSN: 1932-6203
Popis: Background: Antibodies that protect against Plasmodium falciparum (Pf) malaria are only acquired after years of repeated infections. The B cell biology that underlies this observation is poorly understood. We previously reported that ‘‘atypical’’ memory B cells are increased in children and adults exposed to intense Pf transmission in Mali, similar to what has been observed in individuals infected with HIV. In this study we examined B cell subsets of Pf -infected adults in Peru and Mali to determine if Pf transmission intensity correlates with atypical memory B cell expansion. Methodology/Principal Findings: In this cross-sectional study venous blood was collected from adults in areas of zero (U.S., n=10), low (Peru, n=18) and high (Mali, n=12) Pf transmission. Adults in Peru and Mali were infected with Pf at the time of blood collection. Thawed lymphocytes were analyzed by flow cytometry to quantify B cell subsets, including atypical memory B cells, defined by the cell surface markers CD19 + CD20 + CD21 2 CD27 2 CD10 2 . In Peru, the mean level of atypical memory B cells, as a percent of total B cells, was higher than U.S. adults (Peru mean: 5.4% [95% CI: 3.61–7.28]; U.S. mean: 1.4% [95% CI: 0.92–1.81]; p,0.0001) but lower than Malian adults (Mali mean 13.1% [95% CI: 10.68–15.57]; p=0.0001). In Peru, individuals self-reporting >1 prior malaria episodes had a higher percentage of atypical memory B cells compared to those reporting no prior episodes (>1 prior episodes mean: 6.6% [95% CI: 4.09–9.11]; no prior episodes mean: 3.1% [95% CI: 1.52–4.73]; p=0.028). Conclusions/Significance: Compared to Pf-naive controls, atypical memory B cells were increased in Peruvian adults exposed to low Pf transmission, and further increased in Malian adults exposed to intense Pf transmission. Understanding the origin, function and antigen specificity of atypical memory B cells in the context of Pf infection could contribute to our understanding of naturally-acquired malaria immunity.
Databáze: OpenAIRE
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