Enhanced basal autophagy supports ameloblastoma-derived cell survival and reactivation
| Autor: | Olajumoke Ajibola Effiom, Sunday O. Akintoye, Kathleen Boesze-Battaglia, Adetokunbo Babajide Olawuyi, G T Arotiba, Rachel C. Sharp, Anuradha Dhingra, Onatolu Odukoya |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Epithelial-Mesenchymal Transition Cell Survival Odontogenic Tumors Biology Article Epithelium Malignant transformation Ameloblastoma Mice 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Sequestosome-1 Protein Ameloblasts Autophagy medicine Animals Humans Clonogenic assay General Dentistry Mesenchymal stem cell Intracellular Signaling Peptides and Proteins Mesenchymal Stem Cells 030206 dentistry Cell Biology General Medicine medicine.disease Xenograft Model Antitumor Assays Adaptor Proteins Vesicular Transport Disease Models Animal 030104 developmental biology Otorhinolaryngology Cancer research Heterografts Female Neoplasm Recurrence Local Stem cell Carrier Proteins Microtubule-Associated Proteins Solid/Multicystic Ameloblastoma |
| Zdroj: | Archives of Oral Biology. 98:61-67 |
| ISSN: | 0003-9969 |
| Popis: | OBJECTIVES: Ameloblastoma is an aggressive odontogenic jaw neoplasm. Its unlimited growth confers high potential for malignant transformation and recurrence. It is unclear why ameloblastoma is highly recurrent despite surgical resection with a wide margin of normal tissue. While canonical autophagy can be used to degrade and eliminate damaged cellular components, it is also a protective mechanism that provides energy and vital metabolites for cell survival. We used ameloblastoma-derived cells to test the hypothesis that autophagic processes play a role in survival and reactivation of ameloblastoma. METHODS: Primary epithelial (EP-AMCs) and mesenchymal (MS-AMCs) ameloblastoma-derived cells were established from tissue samples of solid multicystic ameloblastoma. Clonogenic capacity and basal autophagic capacity were assessed in ameloblastoma-derived cells relative to human odontoma-derived cells (HODCs) and maxilla-mesenchymal stem cells (MX-MSCs). Ability of ameloblastoma-derived cells to survive and form new ameloblastoma was assessed in mouse tumor xenografts. RESULTS: EP-AMCs were highly clonogenic (p < 0.0001) and demonstrated enhanced basal levels of autophagic proteins microtubule-associated protein 1-light chain 3 (LC3) (p |
| Databáze: | OpenAIRE |
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