Cytokine storm and histopathological findings in 60 cases of COVID-19-related death: from viral load research to immunohistochemical quantification of major players IL-1β, IL-6, IL-15 and TNF-α
| Autor: | Paola Frati, Massimo Martelloni, Letizia Alfieri, Roberto Scendoni, Maurizio Lestani, Stefano D'Errico, Carlo Moreschi, Diana Bonuccelli, Paolo Frisoni, Martina Zanon, Vittorio Fineschi, Alessandro Santurro, Margherita Neri, Rosa Maria Gaudio, Ombretta Turriziani, Mariano Cingolani, Marco Di Paolo, Matteo Marti, Matteo Scopetti |
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| Rok vydání: | 2021 |
| Předmět: |
ARDS
Pathology medicine.medical_specialty Interleukin-1beta RT-PCR Autopsy COVID-19 related-death Cytokines Immunohistochemistry Pneumonia RT-PCR SARS-CoV-2 NO Pathology and Forensic Medicine Proinflammatory cytokine Settore MED/43 - Medicina Legale Humans Medicine Respiratory system Interleukin 6 Interleukin-15 Lung biology Interleukin-6 Tumor Necrosis Factor-alpha SARS-CoV-2 business.industry COVID-19 Endothelial Cells Correction Pneumonia General Medicine Viral Load Autopsy COVID-19 related-death Cytokines Immunohistochemistry medicine.disease medicine.anatomical_structure Interleukin 15 biology.protein Original Article Cytokine Release Syndrome business Cytokine storm |
| Zdroj: | Forensic Science, Medicine, and Pathology |
| ISSN: | 1556-2891 1547-769X |
| DOI: | 10.1007/s12024-021-00414-9 |
| Popis: | This study involves the histological analysis of samples taken during autopsies in cases of COVID-19 related death to evaluate the inflammatory cytokine response and the tissue localization of the virus in various organs. In all the selected cases, SARS-CoV-2 RT-PCR on swabs collected from the upper (nasopharynx and oropharynx) and/or the lower respiratory (trachea and primary bronchi) tracts were positive. Tissue localization of SARS-CoV-2 was detected using antibodies against the nucleoprotein and the spike protein. Overall, we tested the hypothesis that the overexpression of proinflammatory cytokines plays an important role in the development of COVID-19-associated pneumonia by estimating the expression of multiple cytokines (IL-1β, IL-6, IL-10, IL-15, TNF-α, and MCP-1), inflammatory cells (CD4, CD8, CD20, and CD45), and fibrinogen. Immunohistochemical staining showed that endothelial cells expressed IL-1β in lung samples obtained from the COVID-19 group (p |
| Databáze: | OpenAIRE |
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