Effects of Enterococcus faecalis UGRA10 and the enterocin AS-48 against the fish pathogen Lactococcus garvieae. Studies in vitro and in vivo
| Autor: | Lidia Gil-Martínez, Alberto Baños, Juan José Ariza, Manuel Martínez-Bueno, C. Nuñez, J. David García-López, Eva Valdivia |
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| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Trout Antibiotics Administration Oral Microbiology Enterococcus faecalis Cell Line 03 medical and health sciences Fish Diseases Bacteriocin Bacteriocins Lactococcus Toxicity Tests medicine Animals Pathogen Gram-Positive Bacterial Infections 030304 developmental biology 0303 health sciences Microbial Viability biology 030306 microbiology Probiotics Containment of Biohazards biology.organism_classification Animal Feed Coculture Techniques Diet Survival Rate Seafood Lactococcus garvieae Toxicity Rainbow trout Food Science |
| Zdroj: | Food microbiology. 77 |
| ISSN: | 1095-9998 |
| Popis: | The aim of this study was to evaluate the effects of Enterococcus faecalis UGRA10 and its enterocin AS-48 against the fish pathogen Lactococcus garvieae. The minimum bactericidal concentrations of AS-48 against L. garvieae CECT 5807, 5806, and 5274 were 15.62, 15.62, and 7.81 μg/ml respectively. In broth cultures, enterocin at 100, 50, and 25 μg/ml reduced 108 CFU/ml lactococci after 2, 5, and 10 h, respectively. In co-cultures of UGRA10/L. garvieae at a 1/10 CFU/ml ratio, lactococci were eliminated after 24 h. Studies on UGRA10 biosafety and AS-48 toxicity in R1 cells and in rainbow trout have shown a lack of adverse effects from both the strain and bacteriocin. Trout challenged with L. garvieae and UGRA10 administered in diet 30 days before infection had a cumulative survival rate of 50% compared with 0% for control fish. Trout inoculated with the pathogen and treated by regular dipping in AS-48 baths had a survival rate of 60% after 20 days compared with that of untreated fish (0%). These results indicate the protective effect of the UGRA10 strain and the bacteriocin AS-48 against L. garvieae and the potential of these natural products as alternatives to antibiotics for controlling diseases in aquaculture. |
| Databáze: | OpenAIRE |
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