Morphometry and autoradiography of altered rat enamel protein processing due to chronic exposure to fluoride
| Autor: | R. Zhou, A.E. Zaki, D. R. Eisenmann |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Chronic exposure
medicine.medical_specialty Fluorosis Dental Rats Sprague-Dawley Fluorides chemistry.chemical_compound Dental Enamel Proteins stomatognathic system Amelogenesis Internal medicine Sodium fluoride Ameloblasts medicine Protein biosynthesis Animals Secretion Dental Enamel General Dentistry Analysis of Variance Enamel paint Cell Biology General Medicine Rats stomatognathic diseases Endocrinology Secretory protein Otorhinolaryngology chemistry Biochemistry visual_art visual_art.visual_art_medium Autoradiography Female Ameloblast Fluoride |
| Zdroj: | Archives of Oral Biology. 41:739-747 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/s0003-9969(96)00078-7 |
| Popis: | Female Sprague-Dawley rats had 6 weeks of 0 (control), 75 or 100 parts/10 6 sodium fluoride in their drinking water. Whole mandibular incisors were removed, fixed, demineralized and sections prepared for light-microscopic morphometric analysis of dose-related alterations in enamel protein retention. Other rats given 0 and 75 parts/10 6 only (control and experimental groups) were used for autoradiographic evaluation of alterations in enamel protein removal. 35 S-methionine was applied directly over secretory ameloblasts at the end of the fifth week of fluoride exposure. Incisors were removed either 5 or 7 days later and processed for autoradiographic analysis. The results indicated: (1) extended retention of enamel proteins in fluoride-exposed maturation enamel as well as reduced enamel protein synthesis and/or secretion in the secretory stage; (2) negative linear correlation between extended enamel protein retention and reduced enamel protein secretion among groups; and (3) repression of enamel protein removal. The data are also consistent with the concept that the fluoride effect is multifactorial. |
| Databáze: | OpenAIRE |
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