Daily variations of endolymph composition: relationship with the otolith calcification process in trout

Autor: Patrick Payan, Denis Allemand, Gil Borelli, Eric Tambutté, Marielle Guibbolini, H. De Pontual, Sandrine Puverel, N. Mayer-Gostan, Fabrice Priouzeau
Rok vydání: 2003
Předmět:
Electrophoresis
0106 biological sciences
medicine.medical_specialty
Trout
Physiology
Endolymph
Immunoblotting
Aquatic Science
engineering.material
Otolith
01 natural sciences
Calcium Carbonate
Calcification
Otolithic Membrane
03 medical and health sciences
chemistry.chemical_compound
Calcification
Physiologic
Internal medicine
otorhinolaryngologic diseases
medicine
Animals
Solubility
Molecular Biology
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
0303 health sciences
Supersaturation
biology
Chemistry
010604 marine biology & hydrobiology
Aragonite
Carbon Dioxide
medicine.disease
biology.organism_classification
Circadian Rhythm
medicine.anatomical_structure
Endocrinology
Calcium carbonate
Day night cycle
Insect Science
engineering
Animal Science and Zoology
sense organs
Zdroj: Journal of Experimental Biology (0022-0949) (Company of Biologists), 2003-08, Vol. 206, N. 15, P. 2685-2692
ISSN: 1477-9145
0022-0949
DOI: 10.1242/jeb.00479
Popis: SUMMARYIonic and organic parameters of the otolith calcification process in the trout Oncorhynchus mykiss were analysed in plasma and endolymph over the day:night cycle. Plasma pH remained constant and total CO2concentration was significantly lower (by 21%) during the day than at night. Calcifying parameters (total CO2, total calcium concentration) were measured in the proximal and distal endolymphs and were unchanged in the latter during the day:night cycle, but fluctuated in the former. Non-collagenous protein and collagen concentrations in endolymph were higher(1.5- and 10-fold, respectively) during the day than at night. As there was no change in total calcium concentration, we propose that Ca2+increases during the dark period and was maximal by the end of the night when the total CO2 concentration has also increased (by 14%). Measurements of endolymph pH in situ revealed significant differences between samples from proximal and distal endolymph (7.38 and 7.87,respectively), but no variation between values obtained during the day and at night. Thus, the saturation state of aragonite (Sa) in the proximal endolymph should fluctuate around unity during the day:night cycle, and CaCO3precipitation should occur when supersaturation is reached. The electrophoretic pattern of proximal endolymph showed variations in both major and minor components. Immunoblotting of endolymph, using a rabbit antiserum raised against the otolith soluble organic matrix revealed an increase in the expression of two proteins (65 kDa and 75 kDa) during the day period. We propose that organic matrix and calcium carbonate deposition on the otolith vary antiphasically: organic matrix deposition begins by the end of the day period, when the concentration of organic precursors is maximal in the endolymph, whereas CaCO3 precipitation starts once the solubility of CaCO3 is exceeded.
Databáze: OpenAIRE
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