Long-chain fatty acid assimilation By rhodopseudomonas sphaeroides
Autor: | Thomas B. Campbell, D R Lueking |
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Rok vydání: | 1983 |
Předmět: |
Linolenic Acids
Linoleic acid Oleic Acids Rhodobacter sphaeroides Microbiology Linoleic Acid Membrane Lipids chemistry.chemical_compound Molecular Biology Phospholipids chemistry.chemical_classification Growth medium biology Fatty Acids Fatty acid biology.organism_classification Cerulenin Oleic acid Linoleic Acids chemistry Biochemistry Fatty Acids Unsaturated lipids (amino acids peptides and proteins) Long chain fatty acid Research Article Oleic Acid Polyunsaturated fatty acid |
Zdroj: | Journal of Bacteriology. 153:782-790 |
ISSN: | 1098-5530 0021-9193 |
Popis: | Exogenously supplied long-chain fatty acids have been shown to markedly alleviate the inhibition of phototrophic growth of cultures of Rhodopseudomonas sphaeroides caused by the antibiotic cerulenin. Monounsaturated and polyunsaturated C18 fatty acids were most effective in relieving growth inhibition mediated by cerulenin. Medium supplementation with saturated fatty acids (C14 to C18) failed to influence the inhibitory effect of cerulenin. The addition of mixtures of unsaturated and saturated fatty acids to the growth medium did not enhance the growth of cerulenin-inhibited cultures above that obtained with individual unsaturated fatty acids as supplements. Resolution and fatty acid analysis of the extractable lipids of R. sphaeroides revealed that exogenously supplied fatty acids were directly incorporated into cellular phospholipids. Cells treated with cerulenin displayed an enrichment in their percentage of total saturated fatty acids irrespective of the presence of exogenous fatty acids. Cerulenin produced comparable inhibitions of the rates of both fatty acid and phospholipid synthesis and was further found to preferentially inhibit unsaturated fatty acid synthesis. |
Databáze: | OpenAIRE |
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