In vitro effect of radiation, antibody to epidermal growth factor receptor and Docetaxel in human head and neck squamous carcinoma cells with mutant P53 and over-expressed EGFR
| Autor: | Ingela Turesson, Juan Castro, Nongnit Laytragoon-Lewin, Mehran Ghaderi, Jan Lundgren, Hasan Ustun, Signe Friesland, Freddi Lewin |
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| Rok vydání: | 2008 |
| Předmět: |
Oncology
Cancer Research medicine.medical_specialty DNA Complementary medicine.medical_treatment Docetaxel Internal medicine Cell Line Tumor medicine Humans Epidermal growth factor receptor Oligonucleotide Array Sequence Analysis Hematology biology Cell Death Cell growth Gene Expression Profiling Cell Cycle Antibodies Monoclonal General Medicine Cell cycle Combined Modality Therapy Squamous carcinoma Radiation therapy ErbB Receptors Gene Expression Regulation Head and Neck Neoplasms Cancer research biology.protein Carcinoma Squamous Cell Taxoids Antibody Tumor Suppressor Protein p53 medicine.drug |
| Zdroj: | Journal of cancer research and clinical oncology. 135(2) |
| ISSN: | 1432-1335 |
| Popis: | Radiotherapy is the most frequently used and cheapest treatment both for curative and palliative purposes in HNSCC. Despite advances in technology and intensive treatments with radiation, only half of the patients are cured. New therapeutic approaches focusing on the molecular mechanism that mediate tumour cell growth or cell death in combination with radiotherapy have been suggested. The effects of radiation, antibody to EGFR and Docetaxel as single treatment or in combinations on HNSCC cells were investigated.The established HNSCC cells with mutant (mt) P53 and over-expressed normal EGFR was used as the in vitro model. Gene expression profile, cell cycle progression and cell death were used as the indication of treatment outcome.With c-DNA microarray of well-characterised functional genes, massive changes in the genes expression of HNSCC were detected. The alterations of gene expression profiles do not have any correlation neither on tumour cell growth nor cell death. HNSCC cells with mt P53 and over-expressed normal EGFR did not response to radiation, anti-EGFR monoclonal antibody and their combination therapy. Effective treatment could be obtained from single therapy with Docetaxel. No additive effects on cell cycle arrest or cell death were seen in the combination of Docetaxel to anti-EGFR antibody, radiation or anti-EGFR antibody + radiation.The c-DNA microarray analysis does not indicate any specific target or treatment effects of HNSCC with mt P53 and over-expressed normal EGFR. Single therapy, target at microtubules might be the most suitable treatment modulation in this tumour type. |
| Databáze: | OpenAIRE |
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