Emergence of Porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences
| Autor: | Kyoung-Jin Yoon, Beverly J. Schmitt, Vickie L. Cooper, Leo Koster, P. C. Gauger, Hai Hoang, Eric R. Burrough, Gregory W. Stevenson, Angela Pillatzki, Mary Lea Killian, Kent Schwartz, Darin M. Madson, Dong Sun |
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| Rok vydání: | 2013 |
| Předmět: |
Diarrhea
Swine Porcine epidemic diarrhoea Virus Disease Outbreaks law.invention Feces law Alphacoronavirus 1 medicine Animals Phylogeny Polymerase chain reaction Swine Diseases General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction Porcine epidemic diarrhea virus Outbreak biology.organism_classification Immunohistochemistry Virology United States Microscopy Electron GenBank DNA Viral medicine.symptom Coronavirus Infections |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 25:649-654 |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/1040638713501675 |
| Popis: | During the 10 days commencing April 29, 2013, the Iowa State University Veterinary Diagnostic Laboratory received the first 4 of many submissions from swine farms experiencing explosive epidemics of diarrhea and vomiting affecting all ages, with 90–95% mortality in suckling pigs. Histology revealed severe atrophy of villi in all segments of the small intestines with occasional villus-epithelial syncytial cells, but testing for rotaviruses and Transmissible gastroenteritis virus ( Alphacoronavirus 1) were negative. Negative-staining electron microscopy of feces revealed coronavirus-like particles and a pan-coronavirus polymerase chain reaction (PCR) designed to amplify a conserved region of the polymerase gene for all members in the family Coronaviridae produced expected 251-bp amplicons. Subsequent sequencing and analysis revealed 99.6–100% identity among the PCR amplicons from the 4 farms and 97–99% identity to the corresponding portion of the polymerase gene of Porcine epidemic diarrhea virus (PEDV) strains, with the highest identity (99%) to strains from China in 2012. Findings were corroborated at National Veterinary Services Laboratories using 2 nested S-gene and 1 nested N-gene PCR tests where the sequenced amplicons also had the highest identity with 2012 China strains. Whole genome sequence for the virus from 2 farms in 2 different states using next-generation sequencing technique was compared to PEDV sequences available in GenBank. The 2013 U.S. PEDV had 96.6–99.5% identity with all known PEDV strains and the highest identity (>99.0%) to some of the 2011–2012 Chinese strains. The nearly simultaneous outbreaks of disease, and high degree of homology (99.6–100%) between the PEDV strains from the 4 unrelated farms, suggests a common source of virus. |
| Databáze: | OpenAIRE |
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