Mannose and galactose as substrates for production of itaconic acid byAspergillus terreus
| Autor: | Badal C. Saha, Gregory J. Kennedy |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Softwood Mannose 01 natural sciences Applied Microbiology and Biotechnology Industrial Microbiology 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Aspergillus terreus Itaconic acid chemistry.chemical_classification biology Strain (chemistry) Galactose Succinates biology.organism_classification Aspergillus Glucose 030104 developmental biology Dicarboxylic acid chemistry Biochemistry Fermentation |
| Zdroj: | Letters in Applied Microbiology. 65:527-533 |
| ISSN: | 0266-8254 |
| DOI: | 10.1111/lam.12810 |
| Popis: | Itaconic acid (IA), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. Softwood has the potential to serve as low cost source of sugars for its production. Effective utilization of all softwood derived sugars such as glucose, mannose and galactose by the fungus for production of IA will lower the cost of its production. In this work, 20 A. terreus strains were evaluated for the first time for IA production from mannose and galactose in shake-flasks at initial pH of 3.1, 33 °C and 200 rpm for 7 days. Strain NRRL1971 possesses the unique ability to produce high concentrations of IA from mannose. It produced 36.4±0.2 g IA from 80 g mannose per liter with a yield of 0.46 g g-1 mannose (highest titer reported so far). This strain has the potential to be used for IA production from softwood. The maximum (1.1±0.2 g) IA was produced by strain DSM 23081 from 80 g galactose per liter utilizing only 9.1±0.3 g. Galactose was not suitable for IA production by these strains. This is the first detailed report on the production of IA from mannose and galactose. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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