Ultrafast pulse shaping modulates perceived visual brightness in living animals
| Autor: | Matteo Montagnese, Ursula Rothlisberger, Pedro Luis Herrera, Adrien Chauvet, Sylvain Hermelin, Luigi Bonacina, Ivan Rodriguez, Geoffrey Gaulier, Cédric Schmidt, Swarnendu Bhattacharyya, Florence Chiodini, Jean-Pierre Wolf, Quentin Dietschi |
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| Přispěvatelé: | Group of Applied Physics [Geneva] (GAP), University of Geneva [Switzerland], Ecole Polytechnique Fédérale de Lausanne (EPFL), Institut Lumière Matière [Villeurbanne] (ILM), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Geneva University Hospitals and University of Geneva, Department of Genetic Medicine and Development [Geneva], Université de Genève (UNIGE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Brightness
Photoisomerization genetic structures Light Phase (waves) Physics::Optics ddc:500.2 02 engineering and technology 010402 general chemistry 01 natural sciences law.invention Mice [SPI]Engineering Sciences [physics] Optics ddc:590 law Animals [CHIM]Chemical Sciences ddc:576.5 Research Articles Applied Physics Physics [PHYS]Physics [physics] Multidisciplinary Chemical Physics business.industry SciAdv r-articles 021001 nanoscience & nanotechnology Laser equipment and supplies Pulse shaping eye diseases 0104 chemical sciences Light intensity Femtosecond sense organs 0210 nano-technology business Ultrashort pulse Research Article |
| Zdroj: | Science Advances Science Advances, American Association for the Advancement of Science (AAAS), 2021, 7 (18), pp.eabe1911. ⟨10.1126/sciadv.abe1911⟩ Science Advances, Vol. 7, No 18 (2021) P. eabe1911 |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.abe1911⟩ |
| Popis: | Vision in living mice is sensitive to femtosecond pulse shaping. Vision is usually assumed to be sensitive to the light intensity and spectrum but not to its spectral phase. However, experiments performed on retinal proteins in solution showed that the first step of vision consists in an ultrafast photoisomerization that can be coherently controlled by shaping the phase of femtosecond laser pulses, especially in the multiphoton interaction regime. The link between these experiments in solution and the biological process allowing vision was not demonstrated. Here, we measure the electric signals fired from the retina of living mice upon femtosecond multipulse and single-pulse light stimulation. Our results show that the electrophysiological signaling is sensitive to the manipulation of the light excitation on a femtosecond time scale. The mechanism relies on multiple interactions with the light pulses close to the conical intersection, like pump-dump (photoisomerization interruption) and pump-repump (reverse isomerization) processes. This interpretation is supported both experimentally and by dynamics simulations. |
| Databáze: | OpenAIRE |
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