Detection of Oxidative Stress Induced by Low- and High-Linear Energy Transfer Radiation in Cultured Human Epithelial Cells
| Autor: | Jeffrey H. Ware, Zhaozong Zhou, Jelena Stewart, Jeremiah J. Donahue, X. Steven Wan, Peter Bloch, Jun Guan, Ann R. Kennedy |
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| Rok vydání: | 2005 |
| Předmět: |
Biophysics
Analytical chemistry Linear energy transfer Radiation Dosage medicine.disease_cause Fluorescence spectroscopy Cell Line chemistry.chemical_compound High Linear Energy Transfer Radiation medicine Humans Fluorometry Linear Energy Transfer Radiology Nuclear Medicine and imaging Breast Fluorescein Radiation Gamma ray Dose-Response Relationship Radiation Epithelial Cells Fluoresceins In vitro Oxidative Stress chemistry Nucleon Oxidative stress |
| Zdroj: | Radiation Research. 163:364-368 |
| ISSN: | 1938-5404 0033-7587 |
| Popis: | A standardized dichlorofluorescin (DCF) fluorometric assay capable of measuring radiation-induced oxidative stress was used to determine the effectiveness of protons and high-mass, high-atomic number (Z) and high-energy (HZE) particles to produce oxidative stress in vitro. Protons were found to be about equally as effective as X rays in the generation of oxidative stress in cultured cells. However, 56Fe-ion beams with energies of 1 GeV/nucleon and 5 GeV/nucleon were less effective than X rays or gamma rays in inducing dichlorofluorescin (DCFH) oxidation. The relatively lower slope values for the dose responses of HZE-particle radiation-induced DCFH oxidation indicate that the sensitivity of the DCF fluorometric assay is probably dependent on the linear energy transfer (LET) of the radiation beam. |
| Databáze: | OpenAIRE |
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