Immunotoxicology and expression of human cytochrome P450 in microorganisms
| Autor: | F. P. Guengerich, P. Valadon, Jean-Charles Gautier, M. Bourdi, C Belloc, Ph. Beaune |
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| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Saccharomyces cerevisiae Ticrynafen Immunotoxicology Toxicology Immune system Cytochrome P-450 Enzyme System medicine Animals Humans Amino Acid Sequence Biotransformation Autoantibodies Bacteria biology Autoantibody Cytochrome P450 biology.organism_classification Dihydralazine Yeast Biochemistry Tienilic acid Microsomes Liver biology.protein Rabbits Chemical and Drug Induced Liver Injury medicine.drug |
| Zdroj: | Toxicology. 82:53-60 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/0300-483x(93)90059-2 |
| Popis: | Drug-induced hepatitis can be caused by an abnormal immunological response. In the case of tienilic acid- and dihydralazine-induced hepatitis, we postulated a scheme in which a P450 produced a reactive metabolite (step 1); this reactive metabolite bound to the P450 producing it (step 2) leading to a neoantigen triggering the immune response (step 3); the autoantibodies produced during the immune response recognized the P450 producing the reactive metabolite (step 4). The use of microorganisms (yeast or bacteria) expressing cloned human P450 helped in proving some steps of this postulated scheme, particularly steps 1 and 4. |
| Databáze: | OpenAIRE |
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