Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization
| Autor: | Brice Moukengue, Jérôme Amiaud, Francois Lamoureux, Camille Jacques, Benjamin Ory, Céline Charrier |
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| Přispěvatelé: | Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes - INSERM U1238] (Phy-Os), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Equipe Labellisée LIGUE 2012 [Nantes], Physiologie neurovégétative - PNV (PNP), Université Paul Cézanne - Aix-Marseille 3-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie de la résorption osseuse et thérapie des tumeurs osseuses primitives, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
MESH: Xenograft Model Antitumor Assays Histology MESH: Cell Line Tumor mRNA [SDV]Life Sciences [q-bio] Chromogenic in situ hybridization In situ hybridization 03 medical and health sciences chemistry.chemical_compound MESH: Gene Expression Profiling 0302 clinical medicine MESH: In Situ Hybridization MESH: Oligonucleotides Bone sarcoma MESH: Reverse Transcriptase Polymerase Chain Reaction Bone cell MESH: Mice Nude Digoxigenin MESH: Animals Locked nucleic acid CISH Bone MESH: Mice miRNA MESH: RNA Messenger LNA probes MESH: Humans Chemistry MESH: Real-Time Polymerase Chain Reaction RT-qPCR MESH: Digoxigenin MESH: DNA DNA MESH: Bone and Bones Molecular biology Reverse transcriptase 3. Good health Primers MESH: Histocytological Preparation Techniques 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis Gene expression MESH: MicroRNAs |
| Zdroj: | Methods in molecular biology Methods in molecular biology, 1914, pp.169-196, 2019, ⟨10.1007/978-1-4939-8997-3_9⟩ Methods in Molecular Biology ISBN: 9781493989966 |
| Popis: | International audience; The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes . |
| Databáze: | OpenAIRE |
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