Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization

Autor: Brice Moukengue, Jérôme Amiaud, Francois Lamoureux, Camille Jacques, Benjamin Ory, Céline Charrier
Přispěvatelé: Sarcomes osseux et remodelage des tissus calcifiés - Phy-Os [Nantes - INSERM U1238] (Phy-Os), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN), Equipe Labellisée LIGUE 2012 [Nantes], Physiologie neurovégétative - PNV (PNP), Université Paul Cézanne - Aix-Marseille 3-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Physiopathologie de la résorption osseuse et thérapie des tumeurs osseuses primitives, Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Bretagne Loire (UBL)-Centre hospitalier universitaire de Nantes (CHU Nantes)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
MESH: Xenograft Model Antitumor Assays
Histology
MESH: Cell Line
Tumor

mRNA
[SDV]Life Sciences [q-bio]
Chromogenic in situ hybridization
In situ hybridization
03 medical and health sciences
chemistry.chemical_compound
MESH: Gene Expression Profiling
0302 clinical medicine
MESH: In Situ Hybridization
MESH: Oligonucleotides
Bone sarcoma
MESH: Reverse Transcriptase Polymerase Chain Reaction
Bone cell
MESH: Mice
Nude

Digoxigenin
MESH: Animals
Locked nucleic acid
CISH
Bone
MESH: Mice
miRNA
MESH: RNA
Messenger

LNA probes
MESH: Humans
Chemistry
MESH: Real-Time Polymerase Chain Reaction
RT-qPCR
MESH: Digoxigenin
MESH: DNA
DNA
MESH: Bone and Bones
Molecular biology
Reverse transcriptase
3. Good health
Primers
MESH: Histocytological Preparation Techniques
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Gene expression
MESH: MicroRNAs
Zdroj: Methods in molecular biology
Methods in molecular biology, 1914, pp.169-196, 2019, ⟨10.1007/978-1-4939-8997-3_9⟩
Methods in Molecular Biology ISBN: 9781493989966
DOI: 10.1007/978-1-4939-8997-3_9⟩
Popis: International audience; The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
Databáze: OpenAIRE