Effects of Amphetamine on Immune-Mediated Lung Inflammatory Response in Rats
| Autor: | A. P. Ligeiro-Oliveira, A.M. Fialho de Araújo, Zilma Lúcia da Silva, João Palermo-Neto, Ricardo Lazzarini, Marcelo Nicolas Muscará, W. Tavares de Lima, G. De Nucci |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Hypothalamo-Hypophyseal System Neuroimmunomodulation Ovalbumin Immunology Down-Regulation Motor Activity Allergic inflammation Leukocyte Count chemistry.chemical_compound Endocrinology Immune system Bone Marrow Corticosterone Leukocytes medicine Animals Enzyme Inhibitors Rats Wistar Amphetamine Sensitization Myelopoiesis medicine.diagnostic_test Metyrapone Endocrine and Autonomic Systems business.industry Pneumonia respiratory system biochemical phenomena metabolism and nutrition Rats Chemotaxis Leukocyte Bronchoalveolar lavage medicine.anatomical_structure Neurology chemistry Bone marrow Inflammation Mediators business Bronchoalveolar Lavage Fluid medicine.drug |
| Zdroj: | Neuroimmunomodulation. 11:181-190 |
| ISSN: | 1423-0216 1021-7401 |
| DOI: | 10.1159/000076767 |
| Popis: | Objective: The present study analyzed the effects of acute amphetamine (AMPH) treatment on immune-mediated lung inflammatory response in rats. Methods: There were four experiments. In the first and second experiments, rats were treated with AMPH (1 mg/kg) or 0.9% NaCl, and locomotor activity (experiment 1) and serum AMPH concentrations (experiment 2) were measured 1 or 12 h after treatment. In the third experiment, rats which were immunized with ovalbumin (OVA) were treated 14 days later with 0.9% NaCl or AMPH (1 mg/kg). Twelve hours after these treatments, all animals were submitted to challenge by 1% OVA inhalation being analyzed afterwards for bronchoalveolar lavage fluid (BAL), peripheral blood and bone marrow cellularity. In the fourth and final experiment, rats were treated and studied as for experiment 3, except that half of the animals within each group were previously treated with metyrapone prior to the OVA challenge. Results: In the non- immunized rats, AMPH treatment induced an increase in locomotor activity synchronized to high serum AMPH concentrations 1 h after, but not 12 h after treatment. In OVA-challenged rats, AMPH treatment decreased the total number of inflammatory cells, recovered in both BAL and peripheral blood and increased the total number of bone marrow cells. These effects, observed 1 day after OVA challenge, were abrogated by previous metyrapone treatment. Conclusion: AMPH treatment changed HPA-axis responsiveness to the stress condition imposed by the OVA challenge decreasing lung and blood leukocytes cellularity most probably via corticosterone actions on bone marrow activity. |
| Databáze: | OpenAIRE |
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