Expression of leukemia/lymphoma-related factor (LRF/POKEMON) in human breast carcinoma and other cancers
| Autor: | Devendra K. Agrawal, Himanshu Aggarwal, Anshu Aggarwal, Mary S. Davey, Richard F. Murphy, William J. Hunter, Edibaldo Silva |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Lymphoma Clinical Biochemistry Breast Neoplasms Biology medicine.disease_cause Article Pathology and Forensic Medicine Breast cancer Zbtb7 Carcinoma Non-Small-Cell Lung Neoplasms medicine Carcinoma Humans Breast RNA Messenger Molecular Biology Leukemia Cancer medicine.disease Immunohistochemistry DNA-Binding Proteins Female Breast disease Breast carcinoma Carcinogenesis Transcription Factors |
| Zdroj: | Experimental and Molecular Pathology. 89:140-148 |
| ISSN: | 0014-4800 |
| Popis: | The POK family of proteins plays an important role in not only embryonic development and cell differentiation, but also in oncogenesis. Leukemia/lymphoma-related factor (LRF) belongs to the POK family of transcriptional repressors and is also known as POK erythroid myeloid ontogenic factor (POKEMON), which binds to short transcripts of HIV-1 (FBI-1) and TTF-1 interacting peptide (TIP21). Its oncogenic role is known only in lymphoma, non-small cell lung carcinoma, and malignant gliomas. The functional expression of LRF in human breast carcinoma has not yet been confirmed. The aim of this study was to investigate and compare the expression of LRF in human breast cancer tissues and other human tumors. The expression of LRF mRNA transcripts and protein was observed in twenty human benign and malignant breast biopsy tissues. Expression of LRF was observed in several formalin-fixed tissues by immunohistochemistry and immunofluorescence. All malignant breast tissues expressed mRNA transcripts and protein for LRF. However, 40% and 15% benign breast biopsy tissues expressed LRF mRNA transcripts and protein, respectively. The overall expression of LRF mRNA transcripts and total protein was significantly more in malignant breast tissues than the benign breast tissues. LRF expression was also observed in the nuclei of human colon, renal, lung, hepatocellular carcinomas and thymoma tumor cells. In general, a significantly higher expression of LRF was seen in malignant tissues than in the corresponding benign or normal tissue. Further studies are warranted to determine the malignant role of LRF in human breast carcinoma. |
| Databáze: | OpenAIRE |
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