Extensive Placental Methylation Profiling in Normal Pregnancies
| Autor: | Luciano Calzari, Carlo Santaniello, Enrico Ferrazzi, Jole Costanza, Silvia M. Sirchia, Davide Gentilini, Luigi Corsaro, Monica Miozzo, Silvia Motta, Silvia Tabano, Tatjana Radaelli, Margherita Camanni, Silvano Bosari, Ornella Rondinone, Laura Fontana, Patrizia Colapietro, Alessio Murgia |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Placenta Biology methylome Catalysis Article Inorganic Chemistry Andrology lcsh:Chemistry LINE-1 Pregnancy medicine Humans normal pregnancies Epigenetics Physical and Theoretical Chemistry placenta birth weight Molecular Biology lcsh:QH301-705.5 Spectroscopy Fetus Principal Component Analysis Organic Chemistry Infant Newborn Trophoblast Molecular Sequence Annotation General Medicine Methylation DNA Methylation medicine.disease Computer Science Applications medicine.anatomical_structure Long Interspersed Nucleotide Elements lcsh:Biology (General) lcsh:QD1-999 Cord blood DNA methylation embryonic structures Female |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 2136, p 2136 (2021) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 22; Issue 4; Pages: 2136 |
| ISSN: | 1422-0067 |
| Popis: | The placental methylation pattern is crucial for the regulation of genes involved in trophoblast invasion and placental development, both key events for fetal growth. We investigated LINE-1 methylation and methylome profiling using a methylation EPIC array and the targeted methylation sequencing of 154 normal, full-term pregnancies, stratified by birth weight percentiles. LINE-1 methylation showed evidence of a more pronounced hypomethylation in small neonates compared with normal and large for gestational age. Genome-wide methylation, performed in two subsets of pregnancies, showed very similar methylation profiles among cord blood samples while placentae from different pregnancies appeared very variable. A unique methylation profile emerged in each placenta, which could represent the sum of adjustments that the placenta made during the pregnancy to preserve the epigenetic homeostasis of the fetus. Investigations into the 1000 most variable sites between cord blood and the placenta showed that promoters and gene bodies that are hypermethylated in the placenta are associated with blood-specific functions, whereas those that are hypomethylated belong mainly to pathways involved in cancer. These features support the functional analogies between a placenta and cancer. Our results, which provide a comprehensive analysis of DNA methylation profiling in the human placenta, suggest that its peculiar dynamicity can be relevant for understanding placental plasticity in response to the environment. |
| Databáze: | OpenAIRE |
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