Deformable mirror-based photoacoustic remote sensing (PARS) microscopy for depth scanning

Autor: Lyazzat Mukhangaliyeva, Samed Kocer, Alkris Warren, Kevan Bell, Marian Boktor, Mustafa Yavuz, Eihab Abdel-Rahman, Parsin Haji Reza
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Biomed Opt Express
Popis: Optically shifting the focal plane to allow depth scanning of delicate biological structures and processes in their natural environment offers an appealing alternative to conventional mechanical scanning. Our technique uses a deformable mirror-based photoacoustic remote sensing microscopy (PARS) with a focus shifting of Δz ∼ 240 µm. We achieve this by integrating a deformable mirror that functions as a varifocal mirror for axial scanning. First, the system's focal shift capability was demonstrated with USAF resolution targets and carbon fiber phantoms, followed by in-vivo visualizations of blood vessels in chicken embryo chorioallantoic membrane (CAM). This work represents an initial step toward developing a non-contact, label-free, and aberration-free PARS imaging system with axial scanning capability.
Databáze: OpenAIRE