Expression of a single betaalpha chain protein of equine LH/CG in milk of transgenic rabbits and its biological activity
| Autor: | Louis-Marie Houdebine, G Le Griec, Celine Viglietta, A Perrin, Colette Galet, Florian Guillou, C.Menck Le Bourhis, M Chopineau, Joe Attal, T Magallon |
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| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty DNA Complementary Protein subunit Biology Biochemistry Chorionic Gonadotropin law.invention Cell Line Animals Genetically Modified Endocrinology law Internal medicine medicine Animals Testosterone Breast Horses Transgenes Receptor Equine chorionic gonadotropin Molecular Biology chemistry.chemical_classification Biological activity Luteinizing Hormone Receptors LH In vitro Recombinant Proteins Rats Protein Subunits Milk chemistry Recombinant DNA Female Rabbits Luteinizing hormone Glycoprotein |
| Zdroj: | Molecular and cellular endocrinology. 174(1-2) |
| ISSN: | 0303-7207 |
| Popis: | Equine chorionic gonadotropin (eCG) is a heavily glycosylated glycoprotein composed of non-covalently linked alpha- and beta-subunits. eCG possesses the particularity to bind to both LH and FSH receptors in species other than horses and to have a prolonged plasma half-life. All these properties make it of utmost interest for livestock fertilization program. Up to now, the only source of eCG is the serum of pregnant mare. Rabbit mammary gland is considered as a system able to produce recombinant glycoproteins in sufficient quantity for pharmaceutical use. Here we described the production of a recombinant single betaalpha chain of eLH/CG in the milk of transgenic rabbit. The construction of a single-chain permits to by-pass the problem of association-dissociation of the subunits. This recombinant hormone is greatly expressed (21.7 mg/l) and presents similar in vitro LH and FSH bioactivities. However, betaalphaeLH/CG shows an extremely rapid clearance (approximately 10 min), which could explain the absence of in vivo biological activity. So the rabbit mammary gland is not appropriate for the production of a recombinant active eLH/CG. |
| Databáze: | OpenAIRE |
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