α-Galactosidases production by Debaryomyces hansenii UFV-1
| Autor: | Vinicio T.S. Coelho, Solimar Gonçalves Machado, Pollyanna Amaral Viana, Gabriela Picollo Maitan, Flávia M. L. Passos, Sebastião Tavares de Rezende, Valéria Monteze Guimarães |
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| Rok vydání: | 2011 |
| Předmět: |
Culture media
Sucrose biology biology.organism_classification Enzyme production Applied Microbiology and Biotechnology Yeast chemistry.chemical_compound α-galactosidase chemistry Biochemistry Galactose Carbon source Debaryomyces hansenii Extracellular Debaryomyces hansenii UFV-1 Yeast extract Raffinose Lactose Food Science Biotechnology |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 2092-6456 1226-7708 |
| Popis: | Extracellular and intracellular α-galactosidases were produced by yeast Debaryomyces hansenii UFV-1 grown on different media with several carbon sources. D. hansenii grown in YP-medium (1% yeast extract and 2% peptone) presented maximum cell mass (8.45 mg/mL) after 36 h of cultivation, with lactose as carbon source, followed by sucrose, glucose, raffinose, and galactose. Higher extracellular and intracellular α-galactosidases activities were observed at 48 h of D. hansenii cultivation in YPmedium containing galactose (0.97 and 5.27 U/mL) and lactose (1.28 and 4.88 U/mL), supporting the evidence for the model of induction for the yeast GAL/MEL regulon, such as described in Sacharomyces cereviseae. |
| Databáze: | OpenAIRE |
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