Advanced Technology for Storage and Transport of Purified DNA from Umbilical Cord Blood Prior to Use in Human Leukocyte Antigens Typing and Whole-Genome Microarray Analysis

Autor: Linda L. Kelley, Mariluz P. Mojica Henshaw, Michael E. Hogan, Jeremy Bullough, Jennifer Morgan, Heather M. Martinez, L. Hammond, P. P. J. Dunn
Rok vydání: 2010
Předmět:
Zdroj: Biopreservation and Biobanking. 8:133-138
ISSN: 1947-5543
1947-5535
DOI: 10.1089/bio.2010.0014
Popis: Two technologies for dry-state, ambient temperature transport of biospecimens were evaluated in this study. Umbilical cord blood (UCB) samples from 4 individuals were transported at ambient temperature using GenPlates, and the DNA recovered was compared with DNA purified directly from granulocytes of the same UCB samples. GenTegra™ DNA tubes were then used to transport the DNA from California to North Carolina and New Zealand, either immediately after drying or following 30 days of storage at 25°C and 76°C. The integrity of the recovered DNA was thoroughly tested using 2 human leukocyte antigens (HLA)-typing techniques (bead array and sequencing), as well as microarray-based whole-genome scanning. HLA-typing results were the same for all samples whether the DNA had been stored for 3 days during transport or 30 days at either 25°C or 76°C. There were no differences in the HLA-typing results of DNA recovered from UCB samples stored in GenPlates compared with DNA extracted directly from granulocytes. Moreover, the microarray analysis revealed call rates of99.5% for every sample, regardless of storage method, with a statistical concordance of 99.99% between the UCB samples stored in GenPlates compared with DNA extracted directly from granulocytes. These results indicate that both GenPlates and GenTegra are viable methods of storing and transporting UCB (stem cell) biospecimens in a dry state. The quality and quantity of DNA recovered using both technologies are sufficient for complex genotyping using a number of different methods.
Databáze: OpenAIRE
Externí odkaz: