Characterization of four Phyllanthus species using liquid chromatography coupled to tandem mass spectrometry
| Autor: | Quezia B. Cass, Ricardo da Fontoura Sprenger |
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| Rok vydání: | 2013 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Phyllanthus Flavonols Electrospray ionization Analytical chemistry Mass spectrometry Tandem mass spectrometry Biochemistry Flavones Analytical Chemistry Tandem Mass Spectrometry chemistry.chemical_classification Aqueous solution Chromatography biology Plant Extracts Organic Chemistry General Medicine biology.organism_classification Hydrolyzable Tannins chemistry Ion trap Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 1291:97-103 |
| ISSN: | 0021-9673 |
| Popis: | This paper reports a comparison of four Phyllanthus species (P. amarus, P. stipulatus, P. niruri and P. tenellus), commonly known as stone breaker, by the characterization of the chemical profile of their aqueous extracts. Such characterization was carried out using liquid chromatography coupled to ion trap tandem mass spectrometry (LC–IT-MSn) under reversed-phase gradient elution mode. The results of MS/MS and MS3 on-line experiments, using the electrospray ionization source in the positive and negative mode, are extensively discussed. Furthermore, quercetin-3-O-β- d -glucuronopyranoside was isolated in multimilligram scale from the aqueous extract of P. stipulatus and characterized by mass spectrometry and NMR. Although it is an unusual flavonol in natural products, LC–IT-MSn experiments showed it to be present also in P. amarus. |
| Databáze: | OpenAIRE |
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